This review is based on a webinar I presented for the International Society for Immunohistochemistry and Molecular Morphology (ISIMM) in February 2022. It is intended that all ISIMM webinars will ultimately be published in AIMM as review articles. This work is also dedicated to Clive Taylor, who has deeply impacted my career. It presents a molecularly informed, pattern-based approach to p53 immunohistochemistry interpretation, methodological considerations (ie, antibody selection, optimization, validation, controls, and external quality assessment), and pan-cancer diagnostic applications, including those drawn from gastrointestinal, genitourinary, gynecological, neuroendocrine, hematologic, and neuropathology. It intends to prove the thesis statement that p53 is an exemplar next-generation immunohistochemical marker "born" ahead of its time.

中文翻译：

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p53 作为下一代免疫组织化学标记的范例：分子信息、基于模式的方法、方法学考虑和泛癌症诊断应用。

本综述基于我于 2022 年 2 月向国际免疫组织化学和分子形态学学会 (ISIMM) 举办的网络研讨会。所有 ISIMM 网络研讨会最终都将作为综述文章在 AIMM 上发表。谨以此作品献给对我的职业生涯影响深远的克莱夫·泰勒 (Clive Taylor)。它提出了一种基于分子信息的、基于模式的 p53 免疫组织化学解释方法、方法学考虑因素（即抗体选择、优化、验证、控制和外部质量评估）以及泛癌诊断应用，包括来自胃肠道、泌尿生殖系统、妇科、神经内分泌、血液学和神经病理学。它旨在证明 p53 是超前“诞生”的下一代免疫组化标志物范例的论点。