Raoultella planticola harboring genes that confer resistance to antimicrobials, such as carbapenems, have been associated with severe infections in immunocompromised patients. In this study, we reported the first whole genome sequence of a Brazilian isolate of R. planticola and the genomic context of antibiotic resistance markers. By whole-genome sequencing (WGS) of a carbapenem-resistant R. planticola isolate, RpHUM1, we found 23 resistance-encoding genes belonging to 9 classes of antibiotics (aminoglycosides, β-lactams, fluoroquinolones, fosfomycin, macrolides, phenicols, sulfonamides, tetracycline, and diaminopyrimidine derivatives) and 3 plasmids (RpHUM1pEaer-4382s, RpHUM1_pFDAARGOS_440, and RpHUM1pRSF1010). This isolate coharbored the genes blaKPC-2, which is carried by the plasmid RpHUM1pEaer-4382s, and blaNDM-1 and blaCTX-M-15 all located in the accessory genome. In addition, these genes were associated with, at least, one mobile genetic element. This comprehensive knowledge is of great importance for implementation of control measures to prevent the rapid dissemination of this neglected microorganism and their genetic resistance background.

中文翻译：

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对含有 blaKPC-2、blaNDM-1 和 blaCTX-M-15 的广泛耐药 Raoultella planticola 分离株进行全基因组测序。

Raoultella planticola 含有对碳青霉烯类等抗菌药物产生耐药性的基因，与免疫功能低下患者的严重感染有关。在这项研究中，我们报告了巴西 R. planticola 分离株的第一个全基因组序列和抗生素抗性标记的基因组背景。通过对碳青霉烯类抗生素耐药的 R. planticola 菌株 RpHUM1 进行全基因组测序 (WGS)，我们发现了属于 9 类抗生素（氨基糖苷类、β-内酰胺类、氟喹诺酮类、磷霉素类、大环内酯类、苯酚类、磺胺类、四环素和二氨基嘧啶衍生物）和 3 个质粒（RpHUM1pEaer-4382s、RpHUM1_pFDAARGOS_440 和 RpHUM1pRSF1010）。该分离株共有基因 blaKPC-2（由质粒 RpHUM1pEaer-4382s 携带）以及 blaNDM-1 和 blaCTX-M-15，均位于辅助基因组中。此外，这些基因至少与一种移动遗传元件相关。这种全面的知识对于实施控制措施以防止这种被忽视的微生物及其遗传抗性背景的快速传播具有重要意义。