Traditionally, in vitro oocyte and embryo culture progresses through a series of varying culture medium. To investigate simplifying the in vitro production of bovine cumulus–oocyte complexes (COCs), this study used synthetic oviductal fluid (SOF) supplemented with conjugated linoleic acid (CLA). Special interest was placed on gene expression linked to lipid metabolism and oocyte maturation. COCs were matured in different media: Medium 199 (M199 group), M199 with 100 μM CLA (M199 + CLA group), SOF (SOF group), and SOF with 100 μM CLA (SOF + CLA group). COCs matured with SOF showed a higher relative abundance of mRNA of quality indicators gremlin 1 (GREM1) and prostaglandin-endoperoxide synthase 2 (PTGS2) in oocytes, and GREM1 in cumulus cells compared with in the M199 group. SOF medium COCs had a higher relative abundance of fatty acid desaturase 2 (FADS2) compared with the M199 group, which is essential for lipid metabolism in oocytes. Furthermore, the abundance of stearoyl-coenzyme A desaturase 1 (SCD1) in oocytes matured with SOF was not influenced by the addition of CLA, whereas the relative abundance of SCD1 was reduced in M199 medium with CLA. We concluded that maturation in SOF medium results in a greater abundance of genes linked to quality and lipidic metabolism in oocytes, regardless of the addition of CLA.

传统上，体外卵母细胞和胚胎培养通过一系列不同的培养基进行。为了研究简化牛卵丘-卵母细胞复合物 (COC) 的体外生产，本研究使用补充有共轭亚油酸 (CLA) 的合成输卵管液 (SOF)。人们特别关注与脂质代谢和卵母细胞成熟相关的基因表达。COC在不同培养基中成熟：培养基199（M199组）、含100μM CLA的M199（M199+CLA组）、SOF（SOF组）和含100μM CLA的SOF（SOF+CLA组）。与M199组相比，用SOF成熟的COC显示卵母细胞中质量指标gremlin 1 ( GREM1 )和前列腺素内过氧化物合酶2 ( PTGS2 )以及卵丘细胞中GREM1 mRNA的相对丰度较高。与M199组相比，SOF培养基COC具有较高的脂肪酸去饱和酶2（FADS2 ）相对丰度，这对于卵母细胞的脂质代谢至关重要。此外，用SOF成熟的卵母细胞中硬脂酰辅酶A去饱和酶1( SCD1 )的丰度不受添加CLA的影响，而在含有CLA的M199培养基中SCD1的相对丰度降低。我们得出的结论是，无论是否添加 CLA，SOF 培养基中的成熟都会导致与卵母细胞质量和脂质代谢相关的基因更加丰富。