Anthracnose and stem-end rot are among the most devastating pathogens affecting mangos, leading to significant losses due to rotting during storage. This study aimed to investigate marine strains' potential as biocontrol agents against the postharvest pathogenic fungi Colletotrichum gloeosporioides and Lasiodiplodia theobromae. A total of 415 marine yeast strains isolated from marine environments were evaluated for their antifungal activities against C. gloeosporioides and L. theobromae using a dual culture method. Out of these, 115 yeast strains were found to inhibit the growth of C. gloeosporioides, while 31 strains inhibited the growth of L. theobromae. Among these yeast strains, only eighteen proved effective against both pathogenic fungi. The strains Meyerozyma guilliermondii ARU3232-1 and Pichia kudriavzevii DMKUJC44-2 demonstrated the most significant inhibition of radial mycelial growth. The production of volatile organic compounds (VOCs) showcased an in vitro inhibitory effect on the growth of both C. gloeosporioides and L. theobromae. However, assays testing the production of cellulase, chitinase, pectinase and protease by these antagonistic yeasts yielded negative results. Additionally, there was no observed Fe³⁺ binding competition between the yeast and mold. M. guilliermondii ARU3232-1 showed strong biofilm formation, while P. kudriavzevii DMKUJC44-2 demonstrated moderate biofilm formation. In vivo assays indicated that M. guilliermondii ARU3232-1 reduced anthracnose disease severity by 71 ± 3%, whereas P. kudriavzevii DMKUJC44-2 decreased stem-end rot severity by 61 ± 3%. Therefore, both antagonist marine yeasts have the potential to be used as biocontrol agents against anthracnose and stem-end rot diseases of mango fruits.

炭疽病和茎端腐烂病是影响芒果的最具破坏性的病原体，在储存期间因腐烂而导致重大损失。本研究旨在研究海洋菌株作为生物防治剂对抗采后致病真菌胶孢炭疽菌和可可二孢菌的潜力。使用双重培养方法评估了从海洋环境中分离出的总共 415 株海洋酵母菌株对胶孢梭菌和可可乳杆菌的抗真菌活性。其中，115 株酵母菌株被发现可抑制C. gloeosporioides 的生长，而 31 株酵母菌株可抑制L. theobromae的生长。在这些酵母菌株中，只有十八株被证明对这两种病原真菌有效。菌株Meyerozyma guilliermondii ARU3232-1 和Pichia kudriavzevii DMKUJC44-2 表现出对放射状菌丝体生长最显着的抑制作用。挥发性有机化合物 (VOC) 的产生显示出对C. gloeosporioides和L. theobromae生长的体外抑制作用。然而，对这些拮抗酵母产生纤维素酶、几丁质酶、果胶酶和蛋白酶的检测结果却呈阴性。此外，没有观察到酵母和霉菌之间存在Fe ^{3+结合竞争。}M. guilliermondii ARU3232-1 显示出强烈的生物膜形成，而P. kudriavzevii DMKUJC44-2 显示出中等的生物膜形成。体内测定表明，M. guilliermondii ARU3232-1 将炭疽病的严重程度降低了 71 ± 3%，而P. kudriavzevii DMKUJC44-2 将茎端腐烂的严重程度降低了 61 ± 3%。因此，这两种拮抗海洋酵母都有可能用作芒果炭疽病和茎端腐病的生物防治剂。