Santalum album L. (Santalaceae) is an endangered tree species with high economic potential. To improve its cultivation, the role of strigolactone on the somatic embryogenesis of Santalum leaves was analyzed. Leaves were cultivated in vitro using GR24 (a synthetic analogue of strigolactone) at various concentrations (from 0.1 to 1.0 mg L⁻¹) and meta-Topolin (mT), and integrated microscopy of the somatic embryos was used to describe the structural developments underlying the ontogenesis from the leaf under the influence of GR24. The results showed that Murashige and Skoog medium containing 0.75 mg L⁻¹ GR24 produced a maximum percentage (92.0%) of somatic embryos from the leaf surface and cut ends. Weekly microscopic examinations of cultured tissues showed callogenic primary cell masses and minor vascular bundles along the lamina after 3 wk. The proliferation of these primary cell masses led to the emergence of embryogenic outgrowths from leaf surfaces. The areas undergoing intense cell divisions possessed prominent nuclei. The microscopic examinations confirmed the distinctive developmental stages (globular, heart-shaped, and bipolar structures) of somatic embryos. The highest shoot regeneration frequency (88.9%) and shootlet proliferation were aided by the combined incorporation of 0.5 mg L⁻¹ mT and 0.25 mg L⁻¹ GR24. The regenerated plantlets were rooted and acclimatized successfully under ex vitro conditions with 91.0% survival in field conditions and possessed a normal phenotypic appearance. The described somatic embryogenic protocol can be effectively utilized for large-scale plantlet production, long-term storage, and genetic engineering studies in this multipotent tree species.

檀香科（Santalaceae）是一种具有很高经济潜力的濒危树种。为了改进其栽培，分析了独脚金内酯对檀香叶体细胞胚胎发生的作用。^{使用不同浓度（0.1至1.0 mg L -1} ）的GR24（独脚金内酯的合成类似物）和meta -Topolin ( m T)在体外培养叶子，并使用体细胞胚胎的集成显微镜来描述结构发育GR24 影响下叶子的个体发生。结果表明，含有0.75 mg L ^-1 GR24的Murashige和Skoog培养基从叶表面和切端产生最大百分比（92.0%）的体细胞胚。培养组织的每周显微镜检查显示3周后沿层板出现愈伤组织原代细胞团和小维管束。这些原代细胞团的增殖导致叶表面出现胚胎生长。经历剧烈细胞分裂的区域具有突出的细胞核。显微镜检查证实了体细胞胚胎的独特发育阶段（球形、心形和双极结构）。^{0.5 mg L -1} m T 和 0.25 mg L ^-1 GR24的联合掺入有助于最高的芽再生频率 (88.9%) 和芽增殖。再生植株在体外条件下成功生根并适应环境，田间条件下成活率为91.0%，并具有正常的表型外观。所描述的体细胞胚胎发生协议可以有效地用于这种多能树种的大规模苗生产、长期储存和基因工程研究。