Staphylococcus aureus (S. aureus), a bacterium that causes staphylococcal food poisoning, is a Gram-positive human pathogen commonly found in the environment, as well as in the nose and on the skin of humans. Conventional detection methods for this bacterium involve bacterial counting and polymerase chain reaction (PCR), which are constrained by slow processing times and expensive equipment. This study reveals a promising functionalization of gold-interdigitated single-wave-shaped electrodes (Au-ISWE) with a self-assembled monolayer (SAM) to detect S. aureus with enhanced selectivity, label-free detection, cost-effectiveness, and rapid response. The Au-ISWE bioactive surface consisting of a Cr/Au-featured SiO₂ substrate was fabricated using a SAM of 6-mercaptohexanoic acid (MHA) to form 6-MHA/EDC-NHS/anti-S. aureus antibodies. The anti-S. aureus antibodies were immobilized on the surface of the ISWE using layer-by-layer interface self-assembly chemistry. Under optimal conditions, this sensing platform was electrochemically characterized, and its limit of detection (LOD) was measured using electrochemical impedance spectroscopy (EIS). The results of this analytical study demonstrate that this platform provides the desired electromechanical microelectrodes for anti-S. aureus antibody immobilization, which exhibits amplified impedance, enabling a wide detection range (10 to 10⁶ CFU mL⁻¹), a low LOD (10 CFU mL⁻¹) within 30 min of response time, good linearity, and high sensitivity. Remarkably, the developed sensor showed a selectivity against different bacteria including B. cereus (Gram-positive bacteria) and E. coli (Gram-negative bacteria). Additionally, it exhibited a stable performance for 21 days at 4 °C, as confirmed by a stability test (approximately 97.3% of its activity retained). Finally, the results obtained using this sensing platform outperformed compared with those obtained using the standard PCR method.

金黄色葡萄球菌（S. aureus）是一种引起葡萄球菌食物中毒的细菌，是一种革兰氏阳性人类病原体，常见于环境以及人类的鼻子和皮肤中。这种细菌的传统检测方法包括细菌计数和聚合酶链反应（PCR），但受到处理时间慢和设备昂贵的限制。这项研究揭示了具有自组装单层 (SAM) 的金交指单波形电极 (Au-ISWE) 的一种有前景的功能化，可用于检测金黄色葡萄球菌，具有增强的选择性、无标记检测、成本效益和快速检测功能。回复。使用6-巯基己酸(MHA)的SAM来制造由Cr/Au特征的SiO ₂基底组成的Au-ISWE生物活性表面以形成6-MHA/EDC-NHS/抗金黄色葡萄球菌抗体。使用逐层界面自组装化学将抗金黄色葡萄球菌抗体固定在 ISWE 的表面上。在最佳条件下，对该传感平台进行了电化学表征，并使用电化学阻抗谱 (EIS) 测量了其检测限 (LOD)。这项分析研究的结果表明，该平台为抗金黄色葡萄球菌抗体固定提供了所需的机电微电极，其表现出放大的阻抗，实现了宽检测范围（10至10 ⁶  CFU mL ^-1）、低LOD（10 CFU mL ^-1 ) 响应时间在 30 分钟内，线性好，灵敏度高。值得注意的是，所开发的传感器对不同的细菌表现出选择性，包​​括蜡状芽孢杆菌（革兰氏阳性菌）和大肠杆菌（革兰氏阴性菌）。此外，经稳定性测试证实，它在 4°C 下表现出 21 天的稳定性能（保留了约 97.3% 的活性）。最后，使用该传感平台获得的结果优于使用标准 PCR 方法获得的结果。