Objective: The clinical value of an automatic chromosome harvester was evaluated, which included a comparison between the manual and automatic harvesting for the isolation of amniotic fluid cell chromosomes. Methods: Amniotic fluid samples from 96 high-risk gravida cases identified at 17-25 weeks treated at the Prenatal Diagnostic and Reproductive Center from June to July 2022 were collected. These samples underwent both manual and automatic chromosome collection, and their harvest time and number of amniotic cells were compared. These chromosomes were then used to produce karyotypic data for each sample using an automatic chromosomal karyotype analysis system, scan karyotype. Results: The average automatic harvesting time per sample, 3.92 min, was significantly lower than that of the manual harvesting, 7.89 min (p < 0.001). In addition, the average number of cells from the automatic harvesting (4.16 × 106 pieces) was significantly increased when compared with those of the manual group (2.10 × 106 pieces; p < 0.001). Further karyotyping revealed that both sets of chromosomes produced clear bands and good dispersion data, producing no significant differences in these evaluations (p > 0.05). However, the number of analyzable karyotypes obtained using the automatic harvester was significantly higher than those of the manual harvesting (p < 0.001). Conclusions: The automatic chromosome harvester can effectively save time, manual labor and consumables, harvest more analyzable karyotypes, and improve the efficiency of clinical diagnosis. The automatic chromosome harvester is highly stable and repeatable, which has the potential to help achieve large-scale standardized chromosome harvesting and is worthy of widespread clinical promotion.

中文翻译：

---

评估自动染色体采集在产前核型分析中的临床应用。

目的：评价自动染色体采集器的临床价值，包括手动和自动采集羊水细胞染色体分离的比较。方法：收集2022年6月至2022年7月在产前诊断和生殖中心治疗的96例17-25周高危妊娠病例的羊水样本。这些样本进行了手动和自动染色体收集，并比较了它们的收获时间和羊膜细胞数量。然后使用自动染色体核型分析系统（扫描核型）将这些染色体用于生成每个样本的核型数据。结果：每个样品的平均自动收获时间为 3.92 分钟，显着低于手动收获时间 7.89 分钟 (p < 0.001)。此外，与手动组（2.10 × 106 个；p < 0.001）相比，自动收获的平均细胞数（4.16 × 106 个）显着增加。进一步的核型分析显示，两组染色体都产生清晰的条带和良好的分散数据，在这些评估中没有产生显着差异（p > 0.05）。然而，使用自动收获机获得的可分析核型数量显着高于手动收获机（p < 0.001）。结论：全自动染色体收获机可以有效节省时间、人工和耗材，收获更多可分析的核型，提高临床诊断效率。该全自动染色体收获机具有高度稳定性和重复性，有潜力帮助实现大规模标准化染色体收获，值得临床广泛推广。