The regulation of the intracellular level of ATP is a fundamental aspect of bioenergetics. Actin cytoskeletal dynamics have been reported to be an energetic drain in developing neurons and platelets. We addressed the role of actin dynamics in primary embryonic chicken neurons using luciferase assays, and by measurement of the ATP/ADP ratio using the ratiometric reporter PercevalHR and the ATP level using the ratiometric reporter mRuby-iATPSnFR. None of the methods revealed an effect of suppressing actin dynamics on the decline in the neuronal ATP level or the ATP/ADP ratio following shutdown of ATP production. Similarly, we find that treatments that elevate or suppress actin dynamics do not alter the ATP/ADP ratio in growth cones, the subcellular domain with the highest actin dynamics in developing neurons. Collectively, the data indicate that actin cytoskeletal dynamics are not a significant energy drain in developing neurons and that the ATP/ADP ratio is maintained when energy utilization varies. Discrepancies between prior work and the current data are discussed with emphasis on methodology and interpretation of the data.

中文翻译：

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肌动蛋白细胞骨架动力学不会对生长锥生物能造成能量消耗。

细胞内 ATP 水平的调节是生物能学的一个基本方面。据报道，肌动蛋白细胞骨架动力学是发育中的神经元和血小板的能量消耗。我们使用荧光素酶测定，并使用比率报告器 PercevalHR 测量 ATP/ADP 比率，并使用比率报告器 mRuby-iATPSnFR 测量 ATP 水平，探讨了肌动蛋白动力学在初级胚胎鸡神经元中的作用。这些方法均未揭示抑制肌动蛋白动态对 ATP 产生停止后神经元 ATP 水平或 ATP/ADP 比率下降的影响。同样，我们发现提高或抑制肌动蛋白动态的治疗不会改变生长锥中的 ATP/ADP 比率，生长锥是发育神经元中肌动蛋白动态最高的亚细胞域。总的来说，数据表明肌动蛋白细胞骨架动力学并不是发育中神经元的显着能量消耗，并且当能量利用率变化时 ATP/ADP 比率得以维持。讨论了先前工作与当前数据之间的差异，重点是方法论和数据解释。