Identifying sex-linked markers from genomic data has both theoretical and applied importance, especially in conservation. Yet, few methods and tools exist to detect such markers from Restriction-site-Associated DNA sequencing reads and even fewer tools can identify sex-linked markers from existing genotyped data. Here, we describe a new R function that can identify sex-linked markers in species with partially non-recombining sex chromosomes. We test the accuracy and speed of our function with an example dataset from a species of conservation concern, the White Shark, Carcharodon carcharias. We further compare our method against other approaches and find that our method detects more sex-linked markers that can be reliably mapped to reference genomes. Overall, we provide a conservation and fisheries-relevant tool that can reliably and efficiently assign sex from genetic data in species with a heterogametic sex and we demonstrate its utility by developing a sex-identification PCR test for White Sharks.

从基因组数据中识别性别连锁标记具有理论和应用的重要性，特别是在保护方面。然而，很少有方法和工具可以从限制性位点相关的 DNA 测序读数中检测此类标记，甚至更少的工具可以从现有基因分型数据中识别性别连锁标记。在这里，我们描述了一种新的 R 函数，可以识别具有部分非重组性染色体的物种中的性别连锁标记。我们使用来自受保护物种白鲨、 Carcharodon carcharias的示例数据集来测试函数的准确性和速度。我们进一步将我们的方法与其他方法进行比较，发现我们的方法检测到更多的性别连锁标记，这些标记可以可靠地映射到参考基因组。总体而言，我们提供了一种与保护和渔业相关的工具，可以根据异配性别物种的遗传数据可靠且有效地分配性别，并且我们通过开发白鲨性别识别 PCR 测试来证明其实用性。