Sepsis is a life-threatening problem by organ dysfunction influenced by negative inflammatory responses and stimulated oxidative stress, which most of sepsis patients about 40-60% are accompanied with myocardial injury. Recently, stem cells derived exosomes could effectively apply in the numerous diseases by combined with natural therapeutic agents. In the present investigation, Sweroside functionalized with exosomes to control inflammatory responses by sepsis and significantly proved the function of depreciated myocardial injury-induced by LPS. The sweroside could have effectively delivered to cardiomyocytes cells via exosome carriers. The induced-SMI rats exhibited severe myocardial injury and apoptosis by in vivo experiments and treatment of sweroside-functionalized exosomes (SWO/EX) reassured the phenotypes. Importantly, SWO/EX significantly downregulated the ROS generation in the SMI rat models. The SOD and GSH activity were also suppressed in SMI rat models, and treated models with SWO/EXO could have effective liberating activity in the Rats. Meanwhile, SWO/EXO treated LPS-induced cardiomyocytes displayed that significant reduction of pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α) levels and also increasing cell survival and prevented apoptosis. Thus, we demonstrate that MS-cells derived exosome with sweroside could have effectively impede sepsis-induced myocardial injury. SWO/EX formulations might be applied as a potent therapeutic agent for SMI therapy.

中文翻译：

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用间充质干细胞衍生的外泌体功能化的 Sweroside 通过调节大鼠的氧化应激和细胞凋亡来减轻脓毒症引起的心肌损伤。

脓毒症是由负性炎症反应和刺激性氧化应激影响的器官功能障碍而危及生命的问题，大多数脓毒症患者约40-60%伴有心肌损伤。最近，干细胞衍生的外泌体通过与天然治疗剂结合可以有效地应用于多种疾病。在本研究中，Sweroside 与外泌体一起发挥作用，控制脓毒症引起的炎症反应，并显着证明了 LPS 诱导的心肌损伤的作用。苦菜苷可以通过外泌体载体有效地递送至心肌细胞。通过体内实验，诱导 SMI 的大鼠表现出严重的心肌损伤和细胞凋亡，并且使用 swerside 功能化的外泌体 (SWO/EX) 治疗可以恢复表型。重要的是，SWO/EX 显着下调 SMI 大鼠模型中 ROS 的产生。SMI大鼠模型中的SOD和GSH活性也受到抑制，并且用SWO/EXO处理的模型可以在大鼠中具有有效的释放活性。同时，SWO/EXO 处理的 LPS 诱导的心肌细胞显示，促炎细胞因子（IL-1β、IL-6 和 TNF-α）水平显着降低，并且还增加了细胞存活并防止细胞凋亡。因此，我们证明 MS 细胞衍生的外泌体与 sweroside 可以有效阻止脓毒症引起的心肌损伤。SWO/EX 制剂可用作 SMI 治疗的有效治疗剂。