Marburg Virus is one of the most neglected diseases that dated back to 1967. Prior to the 21st century, the outbreak of the virus was recorded trice, In 1967, 1998 and 2004. Accurate and early detection of pathogenic viruses such as Marburg, Ebola, Zika, Human Immunodeficiency Virus (HIV), Dengue, Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-COV-2) etc. is crucial for treatment and prevention. In the Last 2 decades, scientists relied on molecular diagnostic assays which include antigen-antibody and nucleic acid-based testing approaches for the detection of pathogenic viruses, bacteria, fungi and pathogenic surveillances. Despite the widely used of these assays, they are hindered by several factors which includes high cost, the use of expensive tools, long turnaround time, the use of chemicals and the need for trained personnel. In order to counter some of these challenges, scientists developed Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-based biosensor characterized by high specificity. Integration of CRISPR-based biosensor with nanomaterials have shown to increase the performance of the biosensors. Thus, this review provides extensive knowledge on the pathogenicity of Zika and Marburg viruses, conventional diagnosis and the prospect of CRISPR-Cas toolbox in conducting accurate diagnosis of these viruses. The review cover 6 sections which include introduction as section 1, overview of the pathogenicity of Ebola and Marburg viruses in terms of pathology, transmission, number of cases are discussed in section 2, conventional approaches are covered in section 3, CRISPR/Cas systems in both prokaryotic and eukaryotic are overviewed in section 4, the link between CRISPR/Cas system and detection of Ebola and Marburg viruses are presented in section 5, open research and issues are highlighted in section 6 while section 7 covers the concluding remarks.

马尔堡病毒是最容易被忽视的疾病之一，其历史可以追溯到1967年。21世纪之前，该病毒曾在1967年、1998年和2004年三次爆发。准确和早期检测马尔堡病毒、埃博拉病毒、寨卡病毒、人类免疫缺陷病毒（HIV）、登革热、严重急性呼吸系统综合症冠状病毒-2（SARS-COV-2）等对于治疗和预防至关重要。在过去的 20 年中，科学家依靠分子诊断分析（包括抗原抗体和基于核酸的测试方法）来检测病原性病毒、细菌、真菌和病原性监测。尽管这些检测方法得到了广泛应用，但它们受到多种因素的阻碍，包括成本高、使用昂贵的工具、周转时间长、化学品的使用以及需要训练有素的人员。为了应对其中一些挑战，科学家们开发了基于簇规则间隔短回文重复（CRISPR）的具有高特异性的生物传感器。基于 CRISPR 的生物传感器与纳米材料的集成已被证明可以提高生物传感器的性能。因此，这篇综述提供了关于寨卡病毒和马尔堡病毒的致病性、常规诊断以及 CRISPR-Cas 工具箱对这些病毒进行准确诊断的前景的广泛知识。综述涵盖 6 个部分，其中第 1 节介绍，第 2 节讨论埃博拉病毒和马尔堡病毒在病理学、传播、病例数方面的致病性概述，第 3 节讨论常规方法，第 3 节介绍 CRISPR/Cas 系统。第 4 节概述了原核生物和真核生物，第 5 节介绍了 CRISPR/Cas 系统与埃博拉病毒和马尔堡病毒检测之间的联系，第 6 节重点介绍了开放研究和问题，第 7 节介绍了结论性意见。