The mitotic cohesin complex necessary for sister chromatid cohesion and chromatin loop formation shows local and global association to chromosomes in response to DNA double-strand breaks (DSBs). Here, by genome-wide binding analysis of the meiotic cohesin with Rec8, we found that the Rec8-localization profile along chromosomes is altered from middle to late meiotic prophase I with cleavage-independent dissociation. Each Rec8-binding site on the chromosome axis follows a unique alternation pattern with dissociation and probably association. Centromeres showed altered Rec8 binding in late prophase I relative to mid-prophase I, implying chromosome remodeling of the regions. Rec8 dissociation ratio per chromosome is correlated well with meiotic DSB density. Indeed, the spo11 mutant deficient in meiotic DSB formation did not change the distribution of Rec8 along chromosomes in late meiotic prophase I. These suggest the presence of a meiosis-specific regulatory pathway for the global binding of Rec8-cohesin in response to DSBs.

中文翻译：

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DNA双链断裂调节酵母减数分裂过程中Rec8-cohesin的独立于切割的释放

姐妹染色单体粘合和染色质环形成所必需的有丝分裂粘合蛋白复合物显示出与染色体的局部和整体关联，以响应 DNA 双链断裂 (DSB)。在这里，通过减数分裂粘连蛋白与 Rec8 的全基因组结合分析，我们发现沿染色体的 Rec8 定位谱从减数分裂前期 I 中期到晚期发生了变化，并伴随着不依赖于裂解的解离。染色体轴上的每个 Rec8 结合位点都遵循独特的交替模式，包括解离和可能的关联。相对于前期 I 中期，着丝粒在前期 I 晚期显示出 Rec8 结合的改变，这意味着这些区域的染色体重塑。每条染色体的 Rec8 解离率与减数分裂 DSB 密度密切相关。事实上，减数分裂 DSB 形成缺陷的spo11突变体并没有改变减数分裂前期 I 中 Rec8 沿染色体的分布。这表明存在减数分裂特异性调节途径，用于响应 DSB 的 Rec8-cohesin 整体结合。