BACKGROUND Adult-onset immunodeficiency (AOID) is associated with the presence of anti-interferon gamma autoantibodies (anti-IFN-γ autoAbs), which neutralizes IFN-γ signaling and leads to susceptibility to intracellular opportunistic infections. However, measuring neutralizing autoAbs is not practical for general clinical laboratories. OBJECTIVE This study aims to develop a competitive immunochromatographic (IC) strip test for detecting autoAbs that recognize the same epitope as a potent neutralizing antibody. METHODS The competitive IC strip test detecting autoAbs that recognize the same epitope as mouse anti-IFN-γ monoclonal antibody, clone B27 (B27 mAb) was fabricated and used to determine B27 epitope recognizing autoAb (B27 AAb) in AOID plasma. The competitive ELISA was used as a comparative method. AR patients. RESULTS The efficacy of the IC strip test was compared with competitive ELISA and found a percent positive agreement of 91.30%, a percent negative agreement of 79.31%, and a percent overall agreement of 84.62%. CONCLUSIONS The results from the competitive IC strip test were consistent with those from competitive ELISA, indicating that the generated IC strip could detect B27 AAb in AOID plasma.

中文翻译：

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开发免疫层析试纸条测试，用于快速检测成人发病的免疫缺陷中的有效中和性抗干扰素γ抗体。

背景成人发病的免疫缺陷（AOID）与抗干扰素γ自身抗体（抗IFN-γ自身抗体）的存在有关，该抗体中和IFN-γ信号传导并导致对细胞内机会性感染的易感性。然而，测量中和性自身抗体对于一般临床实验室来说并不实用。目的 本研究旨在开发一种竞争性免疫层析 (IC) 试纸条测试，用于检测可识别与有效中和抗体相同表位的自身抗体。方法 制备竞争性 IC 试纸条测试，检测与小鼠抗 IFN-γ 单克隆抗体克隆 B27 (B27 mAb) 识别相同表位的 autoAb，并用于测定 AOID 血浆中识别 B27 表位的 autoAb (B27 AAb)。使用竞争性ELISA作为比较方法。AR患者。结果 将 IC 试纸条测试的功效与竞争性 ELISA 进行比较，发现阳性一致性百分比为 91.30%，阴性一致性百分比为 79.31%，总体一致性百分比为 84.62%。结论 竞争性IC试纸条测试结果与竞争性ELISA结果一致，表明所生成的IC试纸条可以检测AOID血浆中的B27AAb。