Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has important implications in biomedicine and biotechnology. Here we present a rapid, versatile and single-step multicolor flow cytometry method for evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allows simultaneous detection of cell surface glycans with a 10-fold reduction in the number of cells required compared to traditional multistep lectin staining methods. Interestingly, we used this one-step lectin array coupled with dimension reduction algorithms in a proof-of-concept application for discrimination among different tumor and immune cell populations. Moreover, this procedure was also able to unveil T, B and myeloid cell sub-clusters exhibiting differential glycophenotypes. Thus, we report a rapid and versatile lectin cytometry method to simultaneously detect a particular repertoire of surface glycans on living cells that can be easily implemented in different laboratories and core facilities.

中文翻译：

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一种使用荧光染料缀合的凝集素同时检测细胞表面聚糖谱的单步、快速且通用的方法。

细胞表面聚糖在多种生理和病理过程中发挥着重要作用，其评估对生物医学和生物技术具有重要意义。在这里，我们提出了一种快速、多功能和单步多色流式细胞术方法，用于使用一组选定的荧光染料缀合的凝集素评估细胞表面聚糖特征。与传统的多步凝集素染色方法相比，该程序可以同时检测细胞表面聚糖，所需细胞数量减少 10 倍。有趣的是，我们在概念验证应用中使用了这种一步式凝集素阵列与降维算法，以区分不同的肿瘤和免疫细胞群体。此外，该程序还能够揭示表现出不同糖表型的 T、B 和骨髓细胞亚群。因此，我们报告了一种快速且通用的凝集素细胞计数方法，可以同时检测活细胞上特定的表面聚糖，该方法可以在不同的实验室和核心设施中轻松实施。