Magnaporthe oryzae is a plant pathogenic fungus and model organism due to its agricultural impact and tractability that causes severe blast disease affecting rice. Due to its complex cryptic species, VNTRs and complex polyphyletic lineage due to the presence of many transposable elements. In the present study the internal transcribed spacers (ITS) of the ribosomal DNA sequences were used to characterize the 40 monoconidial M. oryzae strains from blast disease samples of the Telangana. Using DNAsp, performed a population genetics study on the sequencing data and discovered that rDNA sequence variation was highly polymorphic, with 0.01521 nucleotide diversity. In the present investigation 8 haplotype groups with Haplotype 2 (Hap_2) were reported to have with a broader genetic base. With a D value of −2.660 and a P 0.05 Tajima's neutrality test, the haplotype diversity is 0.52317, implying that there are variations in the pathogen isolates' sequences. The pathogen's apparent clonal reproductive behaviour was in contrast to the observed population structure, which suggested that genetic recombination may still be occurring within the pathogen population. The total number of variable sites (S) and mutations (Eta) were 63 and 73 respectively. Further, a haplotype network was constructed for the comparison of genealogical relationship's among (ITS) allele's using Population analysis with reticulate trees (PopArt software) a population genetics software to visualize the broader genetic base and genetic drift among the samples collected from the various locations of Telangana.

稻瘟病菌是一种植物病原真菌和模式生物，由于其对农业的影响和易驯化性，可引起影响水稻的严重稻瘟病。由于其复杂的隐性物种、VNTR 和由于许多转座元件的存在而导致的复杂的多系谱系。在本研究中，核糖体 DNA 序列的内转录间隔区 (ITS) 用于表征来自 Telangana 稻瘟病样本的40 个单分生孢子米霉菌株。利用DNAsp对测序数据进行群体遗传学研究，发现rDNA序列变异具有高度多态性，核苷酸多样性为0.01521。在本次调查中，据报道，单倍型 2 (Hap_2) 的 8 个单倍型组具有更广泛的遗传基础。D值为-2.660，P=0.05田岛中性检验，单倍型多样性为0.52317，表明病原体分离株的序列存在变异。病原体明显的克隆繁殖行为与观察到的种群结构形成鲜明对比，这表明病原体种群内可能仍在发生基因重组。可变位点(S)和突变(Eta)总数分别为63和73。此外，使用网状树群体分析（PopArt软件）构建了一个单倍型网络，用于比较（ITS）等位基因之间的谱系关系，这是一种群体遗传学软件，可以可视化从不同位置收集的样本之间更广泛的遗传基础和遗传漂变。特兰加纳。