The lethal heat treatment dose (time and temperature) for the prepupal life stage of Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), emerald ash borer (EAB), was determined through an in vitro application using a carefully calibrated heat treatment apparatus. The lethal and sublethal effects of heat on A. planipennis prepupae were assessed through a ramped heat delivery application, simulating industrial kilns and conventional heat chamber operations, for treatments combining target temperatures of 54 °C, 55 °C, and 56 °C, and exposure durations of 0 min (i.e., kiln temperature ramp only), 15 min, or 30 min. Prepupal EAB larvae did not survive exposure to 56 °C for 15 min or longer, or to 55 °C for 30 min. Sublethal effects were observed for all other treatments. Sublethal effects included delayed development and failure to complete the pupal and adult life stages.

中文翻译：

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使用 Humble 水浴测试 Agrilus planipennis（鞘翅目：Buprestidae）预蛹的热处理剂量

通过使用仔细校准的热处理设备进行体外应用，确定了白蜡螟 (EAB) 的 Agrilus planipennis Fairmaire（鞘翅目：Buprestidae）蛹前生命阶段的致死热处理剂量（时间和温度）。通过斜坡热传递应用，模拟工业窑炉和传统加热室操作，结合 54 °C、55 °C 和 56 °C 的目标温度进行处理，评估热对 A. planipennis prepupae 的致死和亚致死影响，以及暴露持续时间为 0 分钟（即仅窑炉升温）、15 分钟或 30 分钟。预蛹 EAB 幼虫在 56 °C 下暴露 15 分钟或更长时间，或在 55 °C 下暴露 30 分钟无法存活。所有其他治疗均观察到亚致死效应。亚致死效应包括发育迟缓以及无法完成蛹和成虫生命阶段。