Glioblastoma multiform (GBM) is the most common primary brain tumor with a poor prognosis and few therapeutic choices. In vivo, tumor models are useful for enhancing knowledge of underlying GBM pathology and developing more effective therapies/agents at the preclinical level, as they recapitulate human brain tumors. The C6 glioma cell line has been one of the most widely used cell lines in neuro-oncology research as they produce tumors that share the most similarities with human GBM regarding genetic, invasion, and expansion profiles and characteristics. This review provides an overview of the distinctive features and the different animal models produced by the C6 cell line. We also highlight specific applications of various C6 in vivo models according to the purpose of the study and offer some technical notes for more convenient/repeatable modeling. This work also includes novel findings discovered in our laboratory, which would further enhance the feasibility of the model in preclinical GBM investigations.

中文翻译：

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体内 C6 胶质瘤模型：对潜在抗胶质母细胞瘤药物进行更有效的临床前评估的更新和指南。

多形性胶质母细胞瘤（GBM）是最常见的原发性脑肿瘤，预后不良，治疗选择很少。在体内，肿瘤模型可用于增强对潜在 GBM 病理学的了解，并在临床前水平开发更有效的疗法/药剂，因为它们概括了人类脑肿瘤。C6 神经胶质瘤细胞系一直是神经肿瘤学研究中使用最广泛的细胞系之一，因为它们产生的肿瘤在遗传、侵袭和扩张概况和特征方面与人类 GBM 最相似。本综述概述了 C6 细胞系的独特特征和不同的动物模型。我们还根据研究目的重点介绍各种 C6 体内模型的具体应用，并提供一些技术说明以实现更方便/可重复的建模。这项工作还包括我们实验室发现的新发现，这将进一步增强该模型在临床前 GBM 研究中的可行性。