Monitoring phycotoxin accumulation in marine products such as edible shellfish is a regulatory requirement in many countries. Therefore, a simple and rapid onsite quantification method is sought. Herein, we present a fluorescence polarization immunoassay (FPIA), a well-known one-step immunoassay, using a portable fluorescence polarization analyzer for domoic acid (DA), widely referred to as the primary toxin of amnesic shellfish poisoning (ASP). To establish FPIA for DA, the matrix effect of methanol, which is widely used to extract DA from shellfish, on FPIA was investigated. To validate this method, we performed a spike recovery test using oysters containing DA at a concentration equivalent to the regulatory limits of North America and the European Union (20 mg/kg). The recovery rate was found to be 79.4-114.7%, which is equivalent to that of the commercially available enzyme-linked immunosorbent assay (ELISA). We expect that this FPIA system will enable the quantitative onsite analysis of DA and significantly contribute to the safety of marine products.

中文翻译：

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使用便携式分析仪通过荧光偏振免疫分析快速测定海鲜中的软骨藻酸。

监测食用贝类等海产品中藻毒素的积累是许多国家的监管要求。因此，寻求一种简单、快速的现场定量方法。在此，我们提出了一种荧光偏振免疫分析 (FPIA)，这是一种众所周知的一步免疫分析，使用便携式荧光偏振分析仪检测软骨藻酸 (DA)，软骨藻酸被广泛称为遗忘性贝类中毒 (ASP) 的主要毒素。为了建立 DA 的 FPIA，研究了广泛用于从贝类中提取 DA 的甲醇对 FPIA 的基质效应。为了验证该方法，我们使用含有 DA 的牡蛎进行了加标回收率测试，其浓度相当于北美和欧盟的监管限值 (20 mg/kg)。回收率为79.4-114.7%，与市售酶联免疫吸附测定（ELISA）的回收率相当。我们期望该FPIA系统能够实现DA的定量现场分析，并为海洋产品的安全做出重大贡献。