Dugesia hepta and D. benazzii are two species found in Sardinia and Corsica. Previous studies have been unable to resolve their evolutionary relationships and there was doubt about the monophyly of D. benazzii. This study used molecular and morphological data to develop a rigorous species-delimitation procedure within an integrative framework. Three different species discovery methods (ABGD, GMYC, and mPTP) were applied on single-locus data to formulate primary species hypotheses. Secondary species hypotheses were proposed based on two Bayesian species-validation methods (BPP and BFD), morphological, and karyological traits. This study presents evidence supporting the recognition of two new species: Dugesia mariae from Corsica and Dugesia hoidi from Sardinia. A redescription of D. benazzii is also reported. The phylogenetic relationships between these four species were resolved with high support, except for D. hoidi. The performance of the different molecular-delimitation methodologies is discussed, as well as the presence of ciliate parasites in D. benazzii and D. mariae.

中文翻译：

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神奇动物以及如何界定它们：使用多物种合并方法的综合方法揭示了来自科西嘉岛和撒丁岛的两种新的地方性 Dugesia 物种（扁形动物：Tricladida）

Dugesia hepta 和 D. benazzii 是在撒丁岛和科西嘉岛发现的两个物种。先前的研究未能解决它们的进化关系，并且对 D. benazzii 的单系存在怀疑。这项研究使用分子和形态学数据在综合框架内制定了严格的物种界定程序。对单基因座数据应用三种不同的物种发现方法（ABGD、GMYC 和 mPTP）来制定主要物种假设。基于两种贝叶斯物种验证方法（BPP 和 BFD）、形态学和核学特征提出了次要物种假设。这项研究提供了支持识别两个新物种的证据：来自科西嘉岛的 Dugesia mariae 和来自撒丁岛的 Dugesia hoidi。还报告了 D. benazzii 的重新描述。除 D. hoidi 外，这四个物种之间的系统发育关系得到了高度支持。讨论了不同分子定界方法的性能，以及 D. benazzii 和 D. mariae 中纤毛虫寄生虫的存在。