Immunoassay (IA) is currently the most widely used technique for toxicological screening in drug-impaired driving investigations. However, practical limitations in the scope of testing, and the emergence of new psychoactive substances (NPSs), have highlighted the need for alternative approaches, particularly mass spectrometry-based screening. High-resolution mass spectrometry broadens the scope of testing to include NPSs and increases analyte specificity compared to IA. In addition, it provides a platform with increased flexibility and adaptability to incorporate emerging drugs of interest due to the transient drug market. In this study, a comprehensive screening procedure was developed to identify >200 drugs of interest, including cannabinoids and NPSs in whole blood. Supported liquid extraction and liquid chromatography-quadrupole time-of-flight mass spectrometry using All Ions data acquisition were used. The method was validated in accordance with published recommendations, and all compounds of interest were identified at recommended cutoffs for driving under the influence of drug investigations. Cannabinoids, including 11-nor-9-carboxy-∆9-tetrahydrocannabinol, fentanyl analogs, buprenorphine, novel synthetic opioids and synthetic cannabinoids, were identified at low- to sub-nanogram/milliliter concentrations in whole blood using both positive and negative electrospray ionization acquisition methods.

中文翻译：

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使用支持液体萃取和液相色谱-四极杆飞行时间质谱法对血液中的常见滥用药物、新型精神活性物质和大麻素进行全面毒理学筛查。

免疫测定（IA）是目前药物影响驾驶调查中使用最广泛的毒理学筛查技术。然而，测试范围的实际限制以及新型精神活性物质 (NPS) 的出现，凸显了对替代方法的需求，特别是基于质谱的筛查。与 IA 相比，高分辨率质谱法拓宽了测试范围，将 NPS 纳入其中，并提高了分析物的特异性。此外，它还提供了一个具有更高灵活性和适应性的平台，可以整合由于药物市场瞬息万变而感兴趣的新兴药物。在这项研究中，开发了一种全面的筛选程序来识别超过 200 种感兴趣的药物，包括全血中的大麻素和 NPS。使用支持液体萃取和使用全离子数据采集的液相色谱-四极杆飞行时间质谱法。该方法根据已发布的建议进行了验证，并且所有感兴趣的化合物都在药物调查影响下驾驶的建议截止值进行了鉴定。使用正电喷雾电离和负电喷雾电离在全血中鉴定出低至亚纳克/毫升浓度的大麻素，包括 11-正-9-羧基-Δ9-四氢大麻酚、芬太尼类似物、丁丙诺啡、新型合成阿片类药物和合成大麻素。获取方法。