A direct relationship exists between diverse corals and fish farming in Keten Bay, Amami-Oshima, Japan. The release of coral mucus has a significant impact on the microbial activity of surrounding seawater. To obtain a more detailed understanding of biogeochemical cycles in this environment, the effects of coral mucus on the community structure and function of bacteria in surrounding seawater need to be elucidated. We herein used a bromodeoxyuridine approach to investigate the structures and functions of bacterial communities growing close to mucus derived from two different Acropora corals, AC1 and AC2. The alpha diversities of actively growing bacteria (AGB) were lower in mucus-containing seawater than in control seawater and their community structures significantly differed, suggesting that the growth of specific bacteria was modulated by coral mucus. Rhodobacteraceae and Cryomorphaceae species were the most dominant AGB in response to the mucus of Acropora AC1 and AC2, respectively. In contrast, the growth of Actinomarinaceae, Alteromonadaceae, Flavobacteriaceae, and SAR86 clade bacteria was inhibited by coral mucus. The results of a Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt2) ana-lysis suggested that the predicted functions of AGB in mucus-containing seawater differed from those in seawater. These functions were related to the biosynthesis and degradation of the constituents of coral mucus, such as polysaccharides, sugar acids, and aromatic compounds. The present study demonstrated that complex bacterial community structures and functions may be shaped by coral mucus, suggesting that corals foster diverse bacterial communities that enhance the ecological resilience of this fish farming area.

中文翻译：

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响应周围海水中释放的珊瑚粘液的活跃生长的细菌的群落结构和预测功能。

日本奄美大岛科腾湾的多样化珊瑚与鱼类养殖之间存在直接关系。珊瑚粘液的释放对周围海水的微生物活动有重大影响。为了更详细地了解该环境中的生物地球化学循环，需要阐明珊瑚粘液对周围海水中细菌群落结构和功能的影响。我们在此使用溴脱氧尿苷方法来研究生长在两种不同鹿角珊瑚 AC1 和 AC2 粘液附近的细菌群落的结构和功能。含有粘液的海水中活跃生长的细菌（AGB）的α多样性低于对照海水，并且它们的群落结构显着不同，这表明特定细菌的生长受到珊瑚粘液的调节。红杆菌科和冰冻菌科物种分别是对鹿角珊瑚 AC1 和 AC2 粘液做出反应的最主要的 AGB。相比之下，放线菌科、互单胞菌科、黄杆菌科和SAR86分支细菌的生长受到珊瑚粘液的抑制。通过重建未观察状态进行群落系统发育研究 (PICRUSt2) 分析的结果表明，AGB 在含粘液海水中的预测功能与在海水中的功能不同。这些功能与珊瑚粘液成分（如多糖、糖酸和芳香化合物）的生物合成和降解有关。本研究表明，复杂的细菌群落结构和功能可能是由珊瑚粘液塑造的，这表明珊瑚培育了多种细菌群落，从而增强了该养鱼区的生态恢复力。