Understanding the mechanism of detoxification initiation in arthropods after pesticide exposure is crucial. Although the identity of transcription factors that induce and regulate the expression of detoxification genes in response to pesticides is beginning to emerge, whether transcription factors directly interact with xenobiotics is unclear. The findings of this study revealed that a nuclear hormone receptor, Tetranychus cinnabarinus hormone receptor (HR) TcHR96h, regulates the overexpression of the detoxification gene TcGSTm02, which is involved in cyflumetofen resistance. The nuclear translocation of TcHR96h increased after cyflumetofen exposure, suggesting direct binding with cyflumetofen. The direct binding of TcHR96h and cyflumetofen was supported by several independent proteomic assays that quantify interactions with small molecules. Together, this study proposes a model for the initiation of xenobiotic detoxification in a polyphagous agricultural pest. These insights not only provide a better understanding of the mechanisms of xenobiotic detoxification and metabolism in arthropods, but also are crucial in understanding adaptation in polyphagous herbivores.

中文翻译：

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朱砂叶螨 (Boisduval) 的核受体 TcHR96h 启动的解毒途径。

了解节肢动物接触农药后解毒启动的机制至关重要。尽管诱导和调节杀虫剂解毒基因表达的转录因子的身份已经开始出现，但转录因子是否直接与外源物质相互作用尚不清楚。这项研究的结果表明，核激素受体——朱砂叶螨激素受体（HR）TcHR96h，调节解毒基因TcGSTm02的过度表达，该基因参与了氟甲芬耐药性。TcHR96h 的核易位在接触氟虫芬后增加，表明与氟虫芬直接结合。TcHR96h 和 cyflumetofen 的直接结合得到了几个独立的蛋白质组学测定的支持，这些测定量化了与小分子的相互作用。总之，这项研究提出了一种在多食性农业害虫中启动异生物质解毒的模型。这些见解不仅可以更好地理解节肢动物的外源物质解毒和代谢机制，而且对于理解多食性食草动物的适应也至关重要。