Diagnostic value and challenges of BRAF V600E molecular testing and thyroid FNA cytology: a retrospective study from a tertiary institution in southern Hunan Province, China.,Acta Cytologica

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Diagnostic value and challenges of BRAF V600E molecular testing and thyroid FNA cytology: a retrospective study from a tertiary institution in southern Hunan Province, China.
Acta Cytologica ( IF 1.8 ) Pub Date : 2023-09-14 , DOI: 10.1159/000534138
Zhihua Lan ₁ , Fang Yang ₂ , Jing Zhang ₃ , Ya Lan ₄ , Hongtao Li ₃ , Rongfang He ₃

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INTRODUCTION Combined thyroid fine-needle aspiration (FNA) cytology and valine-to-glutamate substitution at codon 600 of B-Raf proto-oncogene, serine/threonine kinase (BRAF V600E) mutation detection are procedure used for diagnosing thyroid nodules in many Chinese tertiary institutions. This retrospective study at our institution aimed to explore the effectiveness and challenges of the combined approach in diagnosing thyroid nodules and the correlation between BRAF V600E mutation status and behavior of papillary thyroid carcinoma. METHODS Thyroid FNA cytology and BRAF V600E mutation detection results were reviewed between November 2020 and July 2022. A total of 623 patients, each of whom underwent thyroidectomy and final pathological examination after FNA cytology diagnosis, were included in the study. The relationship between the BRAF V600E mutational status and pathological parameters was analyzed using the χ2 test. The effectiveness and challenges of FNA cytology alone and the combined procedure were also evaluated based on the final pathology. RESULTS Of 623 patients, 591 were diagnosed with papillary thyroid carcinoma (PTC), of which 456 were positive for the BRAF V600E mutation. It demonstrated near-perfect specificity for identifying PTC, and its incidence rate showed an age-specific curve with a inverted U-shaped distribution. The final pathological examination showed that the combined procedure had a higher sensitivity (83.91%) than FNA cytology alone (63.45%) for distinguishing PTC from other lesions (P < 0.001). Mutational status was associated with a larger maximum tumor diameter (P = 0.003) and a tendency of capsular invasion (P = 0.0542) but possibly unrelated to central lymph node metastasis (P = 0.1846). Nodular goiters accounted for most benign entities initially designated as Bethesda categories III to V. CONCLUSION BRAF V600E mutational analysis complements cytopathology and improves the PTC detection rate in FNA cytology samples due to the high prevalence of the mutation in China. BRAF V600E mutation does not show a statistical correlation with tumor aggressiveness. Morphological pitfalls such as histocyte aggregation, cystic-lining cells in nodular goiters, and oncocytes in Hashimoto's thyroiditis, were overwhelmingly found in BRAF V600E-negative specimens.

更新日期：2023-09-14

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