Ribosomally synthesized and post-translationally modified peptides (RiPPs) represent a diverse superfamily of natural products with immense potential for drug development. This review provides a concise overview of the recent advances in the discovery of RiPP natural products, focusing on rational strategies such as bioactivity guided screening, enzyme or precursor-based genome mining, and biosynthetic engineering. The challenges associated with activating silent biosynthetic gene clusters and the development of elaborate catalytic systems are also discussed. The logical frameworks emerging from these research studies offer valuable insights into RiPP biosynthesis and engineering, paving the way for broader pharmaceutic applications of these peptide natural products.

中文翻译：

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核糖体合成和翻译后修饰肽 (RiPP) 天然产物的发现和工程设计

核糖体合成和翻译后修饰肽 (RiPP) 代表了天然产物的多样化超家族，具有巨大的药物开发潜力。本综述简要概述了 RiPP 天然产物发现的最新进展，重点关注生物活性引导筛选、基于酶或前体的基因组挖掘以及生物合成工程等合理策略。还讨论了与激活沉默生物合成基因簇和复杂催化系统的开发相关的挑战。这些研究得出的逻辑框架为 RiPP 生物合成和工程提供了宝贵的见解，为这些肽天然产物更广泛的制药应用铺平了道路。