More adolescents are coming out as transgender each year and are put on puberty blockers to suppress natal puberty, which is then followed by cross-hormone treatment to achieve puberty of the desired gender. Studies to examine the effects of puberty suppression and virilizing therapy on future reproductive potential among transgender males are lacking. This study used a translational murine in vitro fertilization model to examine the effects of female puberty suppression with depot leuprolide acetate (LA), followed by virilizing therapy with testosterone cypionate (T), on embryologic and pregnancy outcomes. LA effectively inhibited puberty when mice were treated beginning at 3 weeks of age. LA treatment was associated with higher mouse weight but lower ovarian weight. LA-treated mice ovulated developmentally competent eggs in response to gonadotropin administration, albeit at a higher dose than controls. Ovaries from mice treated with LA and T produced oocytes that had morphologically normal meiotic spindles after in vitro maturation and responded to gonadotropin stimulation. Eggs from mice treated with LA and T were fertilizable and produced developmentally competent embryos that led to births of fertile pups. These results suggest that fertility may not be impaired after puberty suppression and cross-hormone therapy for transgender males.

中文翻译：

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青春期抑制后进行睾酮治疗不会损害雌性小鼠的生殖潜力。

每年都有越来越多的青少年公开承认自己是跨性别者，并服用青春期阻滞剂来抑制出生时的青春期，然后进行跨激素治疗，以实现所需性别的青春期。目前还缺乏研究青春期抑制和男性化治疗对跨性别男性未来生殖潜力的影响。本研究使用转化小鼠体外受精模型来检查长效醋酸亮丙瑞林 (LA) 抑制女性青春期，然后使用环戊丙酸睾酮 (T) 进行男性化治疗对胚胎学和妊娠结局的影响。当小鼠从 3 周龄开始接受治疗时，LA 可有效抑制青春期。LA 治疗与小鼠体重增加有关，但卵巢重量降低。经过 LA 处理的小鼠对促性腺激素的施用做出反应，排出具有发育能力的卵子，尽管剂量高于对照组。用 LA 和 T 处理的小鼠卵巢产生的卵母细胞在体外成熟后具有形态正常的减数分裂纺锤体，并对促性腺激素刺激有反应。经过 LA 和 T 处理的小鼠的卵子可以受精，并产生具有发育能力的胚胎，从而产生可育幼崽。这些结果表明，跨性别男性在青春期抑制和交叉激素治疗后，生育能力可能不会受到损害。