The gastropod Pseudosuccinea columella participates in the dissemination of Fasciola hepatica in the environment, acting as the main intermediate host of this parasite in Brazil. The present study sought to elucidate the possible pathogenic effects of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora on P. columella, by evaluating the influence of infection on alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the concentrations of total protein, uric acid, and urea in the snail's hemolymph. For this, the snails were exposed to EPNs for 24 and 48 h, and for each exposure time, 20 snails were dissected after 7, 14 and 21 days for hemolymph collection. The primary findings suggest a significant proteolysis alongside elevated levels of uric acid and urea in P. columella infected individuals. These findings reveal that H. bacteriophora HP88 infection induced serious changes in the snail's metabolism, triggering important deleterious effects.

腹足动物Pseudosuccinea columella参与了肝片形吸虫在环境中的传播，是该寄生虫在巴西的主要中间宿主。本研究旨在通过评估感染对丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的影响以及总细菌浓度，阐明昆虫病原线虫（EPN）异杆菌对柱状小球菌可能的致病作用。蜗牛血淋巴中的蛋白质、尿酸和尿素。为此，将蜗牛暴露于 EPN 24 和 48 小时，并且对于每个暴露时间，在 7、14 和 21 天后解剖 20 只蜗牛以收集血淋巴。主要研究结果表明，小柱假单胞菌感染个体中存在显着的蛋白水解作用，同时尿酸和尿素水平升高。这些发现表明，H. bacteriophora HP88 感染引起了蜗牛新陈代谢的严重变化，引发了重要的有害影响。