INTRODUCTION The present study was to investigate the clinical role of miR-330-3p in acute cerebral infarction (ACI), including its diagnostic and prognostic potential. Preliminary exploration of its target genes was archived by bioinformatics analysis. METHODS miR-330-3p in plasma of the patients with ACI and controls were quantified by real-time quantitative PCR. The one-month prognosis of the ACI patients was evaluated by the Glasgow outcome scale (GOS). The correlation between the plasma levels of miR-330-3p and the GOS scores was tested by Pearson correlation analysis. The receiver operating characteristic (ROC) curves were established based on the expression level of miR-330-3p in different groups. The miR-330-3p-targeting genes were analyzed using Venn diagram, protein-protein interaction network, and Gene Ontology enrichment analysis. RESULTS MiR-330-3p was significantly increased in the plasma of ACI patients compared with that in healthy controls, and ROC curve revealed its diagnostic value for ACI. miR-330-3p was significantly increased in the plasma of patients with poor one-month prognosis compared with those with good one-month prognosis. MiR-330-3p expression was negatively correlated with GOS score, suggesting its potential to predict the one-month prognosis for ACI. One-year survival analysis revealed surviving patients had lower levels of miR-330-3p than the deceased. miR-330-3p was proven to predict the death of patients with ACI. The miR-330-3p-targeting genes were associated with synapse-related Gene Ontology terms. CONCLUSION MiR-330-3p was upregulated in the plasma of patients with ACI, making it a promising diagnostic and prognostic marker for patients with ACI. MiR-330-3p could facilitate synaptic plasticity following cerebral infarction.

中文翻译：

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血浆microRNA-330-3p水平对急性脑梗死的临床意义

引言本研究旨在探讨 miR-330-3p 在急性脑梗死 (ACI) 中的临床作用，包括其诊断和预后潜力。通过生物信息学分析对其靶基因进行了初步探索。方法通过实时定量PCR对ACI患者和对照组血浆中的miR-330-3p进行定量。采用格拉斯哥预后量表（GOS）评估ACI患者的1个月预后。通过Pearson相关分析检验血浆miR-330-3p水平与GOS评分之间的相关性。根据不同组中miR-330-3p的表达水平建立受试者工作特征（ROC）曲线。使用维恩图、蛋白质-蛋白质相互作用网络和基因本体富集分析对 miR-330-3p 靶向基因进行分析。结果 ACI患者血浆中MiR-330-3p较健康对照显着升高，ROC曲线揭示了其对ACI的诊断价值。与1个月预后良好的患者相比，1个月预后不良的患者血浆中miR-330-3p显着升高。MiR-330-3p 表达与 GOS 评分呈负相关，表明其具有预测 ACI 一个月预后的潜力。一年生存分析显示，幸存患者的 miR-330-3p 水平低于死者。miR-330-3p 被证明可以预测 ACI 患者的死亡。miR-330-3p 靶向基因与突触相关基因本体术语相关。结论 MiR-330-3p 在 ACI 患者血浆中表达上调，使其成为 ACI 患者有前途的诊断和预后标志物。MiR-330-3p 可以促进脑梗塞后的突触可塑性。