Studies on the use of natural products to treat cancer are ongoing, and turmeric (Curcuma longa L.), a medicinal crop, is known for various effects including anticancer activity. In this study, the inhibitory effect of C. longa and demethoxycurcumin on cancer cell growth in a colorectal cancer cell line (HCT116) was investigated by using nuclear magnetic resonance (NMR) spectroscopy-based metabolomics. For this analysis, HCT116 cells were treated with doxorubicin (positive control), C. longa extract, or demethoxycurcumin (20, 40, and 60 μM). In the NMR spectra of the HCT116 cell extract, 45 metabolites were identified and quantified. The quantified metabolites were analyzed by biomarker analysis, and significantly changed metabolites were filtered by the area under the curve (AUC) of the receiver operator characteristic (ROC) curve. Multivariate statistical analysis of NMR spectra was conducted to confirm the distribution among groups. Through an S-line plot, it was possible to identify metabolites that contributed to the differences seen in the OPLS-DA score plot. Taken together, the results reveal that C. longa extract induces oxidative stress and changes the energy metabolism in HCT116 cells, and that demethoxycurcumin inhibits the energy metabolism strategy for the survival of cancer cells, escape from immune cells, and cancer cell proliferation, thereby enabling the survival of HCT116 cells.

中文翻译：

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代谢组学揭示姜黄和去甲氧基姜黄素抑制 HCT116 人结肠癌细胞生长

使用天然产品治疗癌症的研究正在进行中，而姜黄 (Curcuma longa L.) 是一种药用作物，以其多种功效（包括抗癌活性）而闻名。在本研究中，通过使用基于核磁共振 (NMR) 光谱的代谢组学研究了 C. longa 和去甲氧基姜黄素对结直肠癌细胞系 (HCT116) 癌细胞生长的抑制作用。对于此分析，HCT116 细胞用阿霉素（阳性对照）、C. longa 提取物或去甲氧基姜黄素（20、40 和 60 μM）处理。在 HCT116 细胞提取物的 NMR 谱中，对 45 种代谢物进行了鉴定和定量。通过生物标志物分析对定量的代谢物进行分析，并通过受试者工作特征（ROC）曲线的曲线下面积（AUC）过滤显着变化的代谢物。对 NMR 谱进行多变量统计分析以确认组间的分布。通过 S 线图，可以识别导致 OPLS-DA 评分图中差异的代谢物。综上所述，结果表明，龙眼提取物会诱导氧化应激并改变 HCT116 细胞的能量代谢，而去甲氧基姜黄素会抑制癌细胞生存、逃避免疫细胞和癌细胞增殖的能量代谢策略，从而使HCT116 细胞的存活。