The current research was carried out to devise a biological strategy to remediate dyes and metal ions from textile wastewater. For this purpose, Citrobacter sp. M41 was isolated from a textile wastewater sample. This strain efficiently decolorized > 90% of the added reactive black-5 (RB5) dye using yeast extract as a C source at 8.5 pH and 30 °C in mineral salt medium containing a mixture of metals (Pb²⁺, Ni²⁺ and Cd²⁺). Moreover, M41 showed >90% decolorization of RB5 beside >80% concurrent removal of hexavalent Chromium [Cr(VI)]. Furthermore, the granulated corncob and its biochar bioaugmented with M41 and filled within packed bed column based bioreactors efficiently (>90%) removed the RB5 and Cr(VI) from a textile wastewater. The M41 bioaugmented corncob biochar resulted into a 60.8%, 57.8% and 68.7% decrease in chemical oxygen demand (COD), electrical conductivity (EC) and total dissolved solids (TDS) of the wastewater, respectively. A phytotoxicity study conducted by using mung bean (Vigna radiata) as a test crop indicated that the treated synthetic wastewater was relatively less toxic as compared with the untreated wastewater. Therefore, it can be concluded that Citrobater sp. M41 is an important bio-resource for treatment of textile effluent polluted with azo dyes and Cr(VI).

目前的研究旨在设计一种生物策略来修复纺织废水中的染料和金属离子。为此，柠檬酸杆菌属。M41 是从纺织废水样品中分离出来的。该菌株使用酵母提取物作为碳源，在 8.5 pH 和 30 °C 下，在含有金属混合物（Pb 2+ ^、 Ni ²⁺和镉²⁺）。此外，M41 对 RB5 的脱色率超过 90%，同时六价铬 [Cr(VI)] 的去除率超过 80%。此外，用 M41 进行生物强化并填充到基于填充床柱的生物反应器中的颗粒状玉米芯及其生物炭可有效 (>90%) 去除纺织废水中的 RB5 和 Cr(VI)。M41生物增强玉米芯生物炭使废水的化学需氧量（COD）、电导率（EC）和总溶解固体（TDS）分别降低60.8%、57.8%和68.7%。以绿豆（Vigna radiata）为试验作物进行的植物毒性研究表明，与未经处理的废水相比，经过处理的合成废水的毒性相对较低。因此，可以得出结论，Citrobater sp。M41是处理偶氮染料和Cr(VI)污染的纺织废水的重要生物资源。