Background Neutrophils continuously migrate into the oral cavity from various sources like gingival crevicular fluid and saliva both in health and in inflammation. The migration of the neutrophils into the various tissues and into the oral cavity occurs when the host microbial interplay tips the balance favoring the initiation of the inflammatory and immune reactions which depending on the amount of the microbial load results in the development of acute and chronic infections in the susceptible host. Aim The present study was designed to quantify and compare the oral salivary neutrophil levels in patients with gingivitis and chronic and aggressive periodontitis as well as in healthy controls, before and after scaling and root planing (SRP) and to compare the difference within the selected study groups. Materials and Methods Forty subjects were classified into four groups, that is, healthy controls, gingivitis, and chronic and aggressive periodontitis. Oral rinse samples were collected using Hank's balanced salt solution from each patient before and after phase I periodontal therapy. Cells in the rinse samples were stained with Acridine orange, and neutrophil counts were carried out using a fluorescence microscope and a hemocytometer. Results Baseline oral salivary neutrophil levels were maximum in the chronic periodontitis group followed by the aggressive group and then the gingivitis group. Oral salivary neutrophil levels also positively correlated to probing pocket depth, plaque index, calculus index, and gingival index in all four study groups. Maximum reduction in the oral salivary neutrophil levels after phase I periodontal therapy was seen in the gingivitis group. Conclusion From our study, we conclude that the oral salivary neutrophil levels decreased significantly after SRP. Estimation of changes in the oral salivary neutrophil levels has the potential to aid in monitoring treatment outcomes. Thus, it suggests that it could be used as a simple, noninvasive laboratory technique to monitor the periodontal status and disease progression.

中文翻译：

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唾液中性粒细胞在牙周健康和疾病中的定量和比较。

背景 在健康和炎症状态下，中性粒细胞不断从各种来源（如龈沟液和唾液）迁移到口腔中。当宿主微生物相互作用打破平衡，有利于引发炎症和免疫反应时，中性粒细胞就会迁移到各种组织和口腔中，这取决于微生物负荷的量，导致急性和慢性感染的发展在易感宿主中。目的 本研究旨在量化和比较牙龈炎、慢性侵袭性牙周炎患者以及健康对照组在洗牙和根面平整 (SRP) 前后的口腔唾液中性粒细胞水平，并比较所选研究中的差异组。材料与方法 40 名受试者被分为四组，即健康对照组、牙龈炎组、慢性侵袭性牙周炎组。在第一阶段牙周治疗之前和之后，使用汉克平衡盐溶液从每位患者收集口腔冲洗样本。冲洗样品中的细胞用吖啶橙染色，并使用荧光显微镜和血细胞计数器进行中性粒细胞计数。结果 慢性牙周炎组的基线口腔唾液中性粒细胞水平最高，其次是侵袭性组，最后是牙龈炎组。在所有四个研究组中，口腔唾液中性粒细胞水平也与探诊深度、菌斑指数、牙石指数和牙龈指数呈正相关。在牙龈炎组中，第一阶段牙周治疗后口腔唾液中性粒细胞水平最大程度降低。结论 根据我们的研究，我们得出结论，SRP 后口腔唾液中性粒细胞水平显着下降。估计口腔唾液中性粒细胞水平的变化有可能有助于监测治疗结果。因此，它表明它可以用作一种简单的、无创的实验室技术来监测牙周状态和疾病进展。