Nitrogen-tungsten co-doped titanium dioxide nanoparticles (W-N-doped TiO2 NPs) are employed for the photocatalytic degradation of environmental pollutants. However, the potential impact of these nanoparticles on the central nervous system remains a subject of concern. This study aimed to evaluate the effects of W-N-doped TiO2 NPs on neurophysiological and biochemical parameters of healthy rat brains, including behavioral monitoring, electroencephalogram analysis, and oxidative stress markers quantification. Intraperitoneal administration of W-N-doped TiO2 NPs to rats revealed abnormal brain electrical activity and an altered sense of balance in the treated rats. The ability of W-N-doped TiO2 NPs to cross the blood–brain barrier and accumulate in the brain leads to oxidative stress damage, supported by the elevated levels of reactive oxygen species (ROS), nitrite concentration, and malondialdehyde levels. Additionally, exposure to W-N-doped TiO2 NPs significantly reduced the antioxidant enzyme levels, such as catalase and superoxide dismutase, impacting a significant decrease in dopamine and acetylcholinesterase within the rat neural tissue. Furthermore, the inflammatory biomarker tumor necrosis factor-alpha and 8-hydroxy 2-deoxyguanosine significantly increased in response to W-N-doped TiO2 NPs. The findings revealed the adverse effects of W-N-doped TiO2 NPs on the electrical activity of rat brains and the altered concentration of various neuro-biomarkers, highlighting their potential neurotoxicity.

中文翻译：

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氮钨共掺杂二氧化钛纳米颗粒诱导的急性神经生化变化

氮钨共掺杂二氧化钛纳米颗粒（WN 掺杂 TiO2 NP）用于光催化降解环境污染物。然而，这些纳米粒子对中枢神经系统的潜在影响仍然令人担忧。本研究旨在评估 WN 掺杂 TiO2 NPs 对健康大鼠大脑神经生理和生化参数的影响，包括行为监测、脑电图分析和氧化应激标记物定量。对大鼠进行腹腔注射 WN 掺杂的 TiO2 纳米粒子后发现，接受治疗的大鼠脑电活动异常，平衡感改变。掺杂 WN 的 TiO2 纳米颗粒能够穿过血脑屏障并在大脑中积聚，从而导致氧化应激损伤，而活性氧 (ROS) 水平、亚硝酸盐浓度和丙二醛水平升高则支持这种损伤。此外，暴露于掺杂WN的TiO2纳米颗粒显着降低了抗氧化酶的水平，例如过氧化氢酶和超氧化物歧化酶，从而导致大鼠神经组织内的多巴胺和乙酰胆碱酯酶显着降低。此外，炎症生物标志物肿瘤坏死因子-α 和 8-羟基 2-脱氧鸟苷对 WN 掺杂的 TiO2 NP 的反应显着增加。研究结果揭示了掺杂 WN 的 TiO2 纳米粒子对大鼠大脑电活动的不利影响以及各种神经生物标志物浓度的改变，突出了它们潜在的神经毒性。