Objectives

Clostridium perfringens is a well-known spore-forming bacterium that can resist the environment. A mixture of volatile fatty acids or thermal treatments can interact with these bacteria. The aim of this study was to evaluate the effects of different volatile fatty acid concentrations and moderate heat treatment on Clostridium perfringens sporulation.

Methods

A pure culture of Clostridium perfringens type A in Duncan Strong medium was treated with a mixture of volatile fatty acids at several concentrations. A thermal treatment was also tested. To evaluate the effects, a double staining method was employed, and treatments on Clostridium perfringens were analysed by flow cytometry.

Results

Moderate heat treatment destroyed vegetative forms but had no effect on sporulating forms. Volatile fatty acids combined with moderate heat treatment inhibited Clostridium perfringens sporulation.

Conclusions

The use of flow cytometry as an original method for evaluating the treatment of Clostridium perfringens is of interest because of its simplicity, short time to obtain results, and the level of information provided on the microbial population (impact on metabolism). A combination of mild treatments (moderate heat treatment + volatile fatty acids) to decrease the Clostridium perfringens concentration when these bacteria sporulate is a very promising finding for inhibiting Clostridium perfringens propagation.

中文翻译：

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流式细胞仪评价挥发性脂肪酸和适度热处理增强产气荚膜梭状芽胞杆菌营养细胞和孢子灭活的叠加效应

目标

产气荚膜梭菌是一种众所周知的能抵抗环境的芽孢细菌。挥发性脂肪酸的混合物或热处理可以与这些细菌相互作用。本研究的目的是评估不同挥发性脂肪酸浓度和适度热处理对产气荚膜梭菌孢子形成的影响。

方法

用多种浓度的挥发性脂肪酸混合物处理 Duncan Strong 培养基中 A 型产气荚膜梭菌的纯培养物。还测试了热处理。为了评估效果，采用双染色方法，并通过流式细胞术分析对产气荚膜梭菌的处理。

结果

适度的热处理破坏了营养体形式，但对孢子形成没有影响。挥发性脂肪酸与适度热处理相结合可抑制产气荚膜梭菌孢子形成。

结论

使用流式细胞术作为评估产气荚膜梭菌治疗的原始方法引起人们的兴趣，因为它简单、获得结果的时间短以及提供的微生物种群信息水平（对代谢的影响）。当产气荚膜梭菌形成孢子时，结合温和处理（适度热处理+挥发性脂肪酸）来降低产气荚膜梭菌浓度，是抑制产气荚膜梭菌繁殖的非常有希望的发现。