Our previous studies on the treatment of spinal cord injuries with Mechanical Tissue Resuscitation (MTR) in rats have demonstrated that it can significantly improve the locomotor recovery and BBB scores. MTR treatment also reduced fluid accumulations by T2-imaging and improved the mean neural fiber number and fiber length in injured sites by fiber tractography. Myelin volume was also significantly preserved by MTR treatment. For further clinical application, a large animal model is necessary to assess this treatment. This study examined the effects of application of MTR on traumatic spinal cord injury in a swine model. Traumatic spinal cord contusion injuries (SCI) in swine were created by controlled pneumatic impact device. Negative pressure at -75 mm Hg was continuously applied to the injured site through open cell silicone manifold for 7 days. In vivo MR imaging for T2 and GRE analysis employed a 3T machine, while a 7T machine was employed for diffusion tensor imaging (DTI) and fiber tractography. Histological HE and Luxol fast blue staining were examined. MTR significantly reduced the mean injured volumes over 46% by T2-imaging in the injured sites from 477.34±146.31 mm3 in non-treated group to 255.99±70.28 mm3 in MTR treated group (P<0.01). It also reduced fluid accumulations by relative T2 signal density in the epicenter of the SCI from 1.62±0.27 in non-treated group to 1.22±0.10 in the MTR treated group (P<0.05). The mean injured tissue volume measured by H&E staining was 303.71±78.21 mm3 in the non-treated group and decreased significantly to 162.16±33.0 mm3 in the MTR treated group (P<0.01). The myelin fiber bundles stained by Luxol blue were preserved much more in the MTR treated group (90±29.71 mm3) than in the non-treated group (33.68±24.99 mm3, P<0.01). The fractional anisotropy (FA) values processed by DTI analysis are increased from 0.203±0.027 in the untreated group to 0.238±0.029 in MTR treatment group (P<0.05). Fiber tractography showings the mean fiber numbers across the impacted area were increased over 112% from 327.0±99.74 in the non-treated group to 694.83±297.86 in the MTR treated group (P<0.05). These results indicates local application of MTR for seven days to spinal cord injury in a swine model decreased tissue injury, reduced tissue edema and preserved more myelin fibers as well as nerve fibers in the injured spinal cord. Keywords: Mechanical tissue resuscitation, Negative pressure treatment, Spinal cord injury, Diffusion tensor imaging, Nerve fiber tractography.

中文翻译：

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在猪脊髓损伤模型中通过机械组织复苏减轻组织损伤。

我们之前对大鼠机械组织复苏（MTR）治疗脊髓损伤的研究表明，它可以显着改善运动恢复和 BBB 评分。MTR 治疗还通过 T2 成像减少了积液，并通过纤维束成像改善了受伤部位的平均神经纤维数量和纤维长度。MTR 治疗也显着保留了髓磷脂体积。为了进一步的临床应用，需要大型动物模型来评估这种治疗方法。本研究探讨了应用 MTR 对猪模型创伤性脊髓损伤的影响。猪的创伤性脊髓挫伤（SCI）是由受控气动冲击装置造成的。通过开孔硅胶歧管对受伤部位持续施加-75毫米汞柱的负压7天。用于 T2 和 GRE 分析的体内 MR 成像采用 3T 机器，而 7T 机器用于扩散张量成像 (DTI) 和纤维束成像。检查组织学 HE 和 Luxol 固蓝染色。通过 T2 成像，MTR 使损伤部位的平均损伤体积显着减少了 46% 以上，从未治疗组的 477.34±146.31 mm3 减少到 MTR 治疗组的 255.99±70.28 mm3（P<0.01）。它还通过相对T2信号密度减少了SCI中心的积液，从未治疗组的1.62±0.27减少到MTR治疗组的1.22±0.10（P<0.05）。H&E染色测量的平均损伤组织体积在未治疗组中为303.71±78.21 mm3，在MTR治疗组中显着下降至162.16±33.0 mm3（P<0.01）。MTR处理组（90±29.71 mm3）Luxol蓝染色的髓磷脂纤维束保存量明显多于未处理组（33.68±24.99 mm3，P<0.01）。DTI分析处理的各向异性分数（FA）值从未治疗组的0.203±0.027增加到MTR治疗组的0.238±0.029（P<0.05）。纤维束造影显示，受影响区域的平均纤维数量增加了 112% 以上，从未治疗组的 327.0±99.74 增加到 MTR 治疗组的 694.83±297.86（P<0.05）。这些结果表明，对猪模型的脊髓损伤局部应用MTR 7天，减少了组织损伤，减少了组织水肿，并在受伤的脊髓中保留了更多的髓磷脂纤维和神经纤维。关键词：机械组织复苏、负压治疗、脊髓损伤、弥散张量成像、神经纤维束成像。