BACKGROUND As a consequence of their eco-friendliness, simplicity and non-toxicity, the fabrication of metal and metal oxide nanoparticles using greener chemistry has been a highly attractive research area over the last decade. AIM In this study focused on the fabrication of silver-Zinc oxide nanocomposite (Ag-ZnO NCs) using Ruta chalepensis leaf extract and evaluating its potential biological activities, against Echinococcus granulosus in an in vitro and in vivo model using BALB/c mice. METHODS In this study, the synthesis of Ag-ZnO NCs was accomplished using local R. chalepensis leaf extracts. The synthesized nanocomposites were identified using UV-Vis, SEM-EDX, XRD, and FTIR. For a short-term assessment of acute toxicity, BALB/c mice were given the prepared NCs orally. Dual sets of mice were also intraperitoneally injected with protoscoleces for secondary echinococcosis infection. Furthermore, a blood compatibility test was carried out on the nanocomposites. RESULTS The synthesized Ag-ZnO NCs presented a surface plasmon peak at 329 and 422 nm. The XRD, SEM, and EDX confirmed the purity of the Ag-ZnO NCs. The FTIR spectra indicated the formation of Ag-ZnO NCs. Compared to the untreated infected mice, the treated-infected animals displayed an alteration in the appearance of the hepatic hydatid cysts from hyaline to whitish cloudy with a rough surface appearance. Lysis of RBCs at various doses of Ag-ZnONCs was significantly less than the positive contro,. CONCLUSION These findings revealed that the Ag-ZnO NCs didn't cause any adverse symptoms and no mortality was observed in all administered groups of mice. The obtained outcomes confirmed that concentrations of up to 40 μg/mL of the bio-fabricated Ag-ZnONCs induced no notable harm to the red blood cells.

中文翻译：

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使用 Ruta chalepensis L. 叶提取物作为有效的杀原头虫和抗包虫包囊剂制备的 Ag/ZnO 纳米复合材料的效率。

背景技术由于其生态友好性、简单性和无毒性，使用绿色化学来制造金属和金属氧化物纳米粒子在过去十年中一直是非常有吸引力的研究领域。目的 本研究的重点是使用芸香叶提取物制备银-氧化锌纳米复合材料 (Ag-ZnO NCs)，并使用 BALB/c 小鼠在体外和体内模型中评估其对抗细粒棘球绦虫的潜在生物活性。方法在本研究中，使用当地的查勒彭西斯叶提取物合成了Ag-ZnO NCs。使用 UV-Vis、SEM-EDX、XRD 和 FTIR 对合成的纳米复合材料进行了鉴定。为了短期评估急性毒性，BALB/c 小鼠口服制备的 NC。双组小鼠还腹腔注射原头节以治疗继发性包虫病感染。此外，对纳米复合材料进行了血液相容性测试。结果合成的Ag-ZnO NCs在329和422 nm处呈现表面等离子体峰。XRD、SEM 和 EDX 证实了 Ag-ZnO NC 的纯度。FTIR 光谱表明 Ag-ZnO NC 的形成。与未经治疗的感染小鼠相比，经过治疗的感染小鼠的肝包虫囊肿外观发生变化，从透明变为白色混浊，表面粗糙。不同剂量的 Ag-ZnONC 下红细胞的裂解显着低于阳性对照。结论 这些结果表明，Ag-ZnO NCs 不会引起任何不良症状，并且在所有给药组的小鼠中均未观察到死亡。获得的结果证实，浓度高达 40 μg/mL 的生物制造 Ag-ZnONCs 不会对红细胞造成明显伤害。