Strawberries, the most economically well-known berry crop, are known for their taste, nutritional value, and antioxidant compounds. Various spatiotemporal or seasonal factors are known to affect the strawberry pollen germination rate; however, determinating which factor most influences the variation in the pollen germination rate remains challenging. This study aimed to determine the optimal pollen germination media and pollen short-term storage methods in the Japanese strawberry cultivars ‘Shindai SUS-1’ and ‘Shindai BS8-9’. Each strawberry pollen was incubated for 3 h in the dark on a solid medium with 1.5% agar and different sucrose and boric acid concentrations. The pollen germination rate was then investigated. The optimal pollen germination medium for both cultivars was 1.5% agar, 10% sucrose, and 0.1% boric acid. The small amount of strawberry pollen used in this study was collected immediately after flowering in the morning. Therefore, we also investigated a method for collecting a large amount of pollen while maintaining pollen activity. For both cultivars, the pollen germination rate for dry pollen, incubated for 24 h with silica gel after the petals and calyx were removed, was the same as that of the fresh pollen collected immediately after flower collection. In addition, dry pollen was more efficient in terms of short-term storage than fresh pollen. For ‘Shindai BS8-9’, the germination rate for dry pollen was also higher than that of the fresh pollen when stored in vacuum at −25, 4, 15, 20, and 25°C for 3 and 7 days.

草莓是最具经济知名度的浆果作物，以其味道、营养价值和抗氧化化合物而闻名。已知各种时空或季节因素会影响草莓花粉的发芽率；然而，确定哪个因素对花粉发芽率的变化影响最大仍然具有挑战性。本研究旨在确定日本草莓品种“Shindai SUS-1”和“Shindai BS8-9”的最佳花粉萌发培养基和花粉短期储存方法。将每个草莓花粉在含有 1.5% 琼脂和不同浓度的蔗糖和硼酸的固体培养基上在黑暗中孵育 3 小时。然后调查花粉发芽率。两个品种的最佳花粉萌发培养基为1.5%琼脂、10%蔗糖和0.1%硼酸。本研究中使用的少量草莓花粉是在早晨开花后立即收集的。因此，我们还研究了一种在保持花粉活性的同时收集大量花粉的方法。对于这两个品种，除去花瓣和花萼后用硅胶孵育24小时的干花粉的花粉萌发率与采花后立即采集的新鲜花粉的萌发率相同。此外，干燥花粉在短期储存方面比新鲜花粉更有效。对于‘Shindai BS8-9’，当在-25、4、15、20和25°C真空储存3天和7天时，干花粉的发芽率也高于新鲜花粉的发芽率。