Cowpea is a high-protein legume that can be grown in many environments. Gamma radiation can modify plant metabolism and growth. An experiment was conducted to determine the effect of different gamma-ray doses on cowpea yield and growth. The results showed that gamma radiation had a significant effect on cowpea yield and growth, with the highest yields and growth rates observed at lower doses. Higher doses had a negative effect on cowpea yield and growth. The study suggests that gamma radiation can be used to improve cowpea yield and growth, but that the optimal dose must be determined for each specific cultivar and environment. Results specified that gamma ray significantly affected growth characters and forage yield. Gamma-ray dose of 50 Gy in M1 and M2 generations gave the highest values for all studied characteristics compared to the control and all other irradiation doses, except the crude fiber, followed by 75 and 100 Gy doses for all characters, except number of tillers plant−1 and crude fiber. Seven combinations of SRAP produced 227 loci with an average polymorphism percentage of 85%. The allele frequency of target loci ranged between 0.29 and 0.60, and the PIC was 0.41–0.50 in range, while the GTS% of SRAP combinations ranged from 36 to 63%. The genetic similarities ranged between − 4 and 30%. Ten primers produced 450 loci with a polymorphism percentage of 88%. The allele frequency of target loci ranged between 0.28 and 0.46. The PIC was 0.40–0.50 in range, and the GTS% of IRAP primers ranged from 29 to 71%. The genetic similarities ranged from 8 to 37%. SRAP and IRAP analysis revealed more than two clusters of treated plants. In silico analysis showed that some SRAP primers could align with genes in cowpea and related genomes. The SRAP-F12R9 primer is unique to Phaseolus vulgaris, while the SRAP-F13R15 and SRAP-F13em1 primers align with genes on different chromosomes in cowpea cultivar Xiabao 2. Gamma ray significantly affected growth characters and forage yield of Cowpew. In silico analysis revealed three SRAP combination primers that could align with some genes along cowpea and related family members’ genomes.

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SRAP 和 IRAP 揭示了受伽马射线照射的豇豆 (Vigna unguiculata L.) 的分子特征和遗传关系

豇豆是一种高蛋白豆类，可以在许多环境中生长。伽马辐射可以改变植物的新陈代谢和生长。进行了一项实验以确定不同伽马射线剂量对豇豆产量和生长的影响。结果表明，伽马辐射对豇豆产量和生长有显着影响，在较低剂量下观察到最高的产量和生长速率。较高剂量对豇豆产量和生长有负面影响。研究表明，伽马辐射可用于提高豇豆产量和生长，但必须针对每个特定品种和环境确定最佳剂量。结果表明，伽马射线显着影响生长特性和饲料产量。与对照和除粗纤维外的所有其他辐射剂量相比，M1 和 M2 代中 50 Gy 的伽马射线剂量为所有研究特性提供了最高值，其次是除分蘖数之外的所有性状的 75 和 100 Gy 剂量植物−1 和粗纤维。SRAP的7个组合产生227个位点，平均多态性百分比为85%。目标位点等位基因频率范围为0.29~0.60，PIC范围为0.41~0.50，SRAP组合的GTS%范围为36~63%。遗传相似度在− 4% 到 30% 之间。10个引物产生450个位点，多态性百分比为88%。目标位点的等位基因频率范围在0.28至0.46之间。PIC 范围为 0.40-0.50，IRAP 引物的 GTS% 范围为 29-71%。遗传相似性范围为 8% 至 37%。SRAP 和 IRAP 分析揭示了超过两簇经过处理的植物。计算机分析表明，一些 SRAP 引物可以与豇豆和相关基因组中的基因进行比对。SRAP-F12R9引物是菜豆特有的，而SRAP-F13R15和SRAP-F13em1引物与豇豆品种夏宝2号不同染色体上的基因比对。伽马射线显着影响豇豆的生长性状和饲草产量。计算机分析揭示了三种 SRAP 组合引物，它们可以与豇豆和相关家族成员基因组中的一些基因对齐。