Microtubule-associated protein 65-1 (MAP65-1) protein plays an essential role in plant cellular dynamics through impacting stabilization of the cytoskeleton by serving as a crosslinker of microtubules. The role of MAP65-1 in plants has been associated with phenotypic outcomes in response to various environmental stresses. The Arabidopsis MAP65-1 (AtMAP65-1) is a known virulence target of plant bacterial pathogens and thus a component of plant immunity. Soybean events were generated that carry transgenic alleles for both AtMAP65-1 and GmMAP65-1, the soybean AtMAP65-1 homolog, under control of cauliflower mosaic virus 35S promoter. Both AtMAP65-1 and GmMAP65-1 transgenic soybeans are more resistant to challenges by the soybean bacterial pathogen Pseudomonas syringae pv. glycinea and the oomycete pathogen Phytophthora sojae, but not the soybean cyst nematode, Heterodera glycines. Soybean plants expressing AtMAP65-1 and GmMAP65-1 also display a tolerance to the herbicidal oryzalin, that has a mode of action to destabilize microtubules. In addition, GmMAP65-1 expressing soybean plants show reduced cytosol ion leakage under freezing conditions hinting that ectopic expression of GmMAP65-1 may enhance cold tolerance in soybean. Taken together, over-expression of AtMAP65-1 and GmMAP65-1 confers tolerance of soybean plants to varieties of biotic and abiotic stresses.

中文翻译：

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微管相关蛋白 65-1 (MAP65-1) 表达增强的大豆事件特征。

微管相关蛋白 65-1 (MAP65-1) 蛋白通过充当微管交联剂影响细胞骨架的稳定性，从而在植物细胞动力学中发挥重要作用。MAP65-1 在植物中的作用与响应各种环境胁迫的表型结果相关。拟南芥 MAP65-1 (AtMAP65-1) 是植物细菌病原体的已知毒力靶标，因此是植物免疫的组成部分。产生的大豆事件携带AtMAP65-1和GmMAP65-1（大豆AtMAP65-1同源物）的转基因等位基因，受花椰菜花叶病毒35S启动子的控制。AtMAP65-1 和 GmMAP65-1 转基因大豆对大豆细菌病原体丁香假单胞菌 (Pseudomonas syringae pv.) 的攻击具有更强的抵抗力。大豆胞囊线虫和卵菌病原体大豆疫霉，但不是大豆胞囊线虫、大豆胞囊线虫。表达 AtMAP65-1 和 GmMAP65-1 的大豆植物也表现出对除草剂安磺灵的耐受性，安磺灵具有破坏微管稳定性的作用模式。此外，表达GmMAP65-1的大豆植物在冷冻条件下表现出细胞质离子渗漏减少，这暗示GmMAP65-1的异位表达可能增强大豆的耐冷性。总之，AtMAP65-1 和 GmMAP65-1 的过度表达赋予大豆植物对各种生物和非生物胁迫的耐受性。