The coronavirus disease 2019 (COVID-19) pandemic has translated into a worldwide economic recession and public health crisis. Bats have been incriminated as the main natural host for the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which is the causative agent of the COVID-19 pandemic. However, the reservoir and carrier hosts of the virus remain unknown. Therefore, a cross sectional serosurvey study was performed to estimate antibodies to SARS-CoV-2. To assess IgM antibodies to SARS-CoV-2 nucleocapsid protein (NP), a SARS-CoV-2 Double Antigen Multispecies diagnostic enzyme-linked immunosorbent assay kit was used. The seropositive samples were confirmed and validated by measuring IgG antibody titers in sera. The enrolled animals were from different locations in the Giza governorate, Egypt, and were sampled at the time of the pandemic; they comprised 92 companion animals and 92 domestic camels. The study established that 4.76% (1/21 clinical samples) of dogs, 7.69% of cats (1/13 shelter samples) and 1.08% (1/92) of camels, had measurable SARS-CoV-2 NP IgM antibodies. All IgM-seropositive samples were IgG positive with a measurable titer of 34.5, 28.6, and 25.8 UI/mL for dog, cat, and camels, respectively. According to our best knowledge, this study was the first to assess SARS-CoV-2 seroprevalence in the specific animals investigated in Egypt. These results may herald a promising epidemiological role for pet animals and camels in SARS-CoV-2 virus maintenance. Thus, our study's results ought to be confirmed with a nationwide seroprevalence study, and further studies are required to clarify whether these animals act as active or passive carriers.

2019 年冠状病毒病 (COVID-19) 大流行已转化为全球经济衰退和公共卫生危机。蝙蝠被认为是严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 的主要自然宿主，它是 COVID-19 大流行的病原体。然而，该病毒的储存宿主和携带宿主仍不清楚。因此，进行了一项横断面血清调查研究来估计 SARS-CoV-2 的抗体。为了评估SARS-CoV-2 核衣壳蛋白 (NP) 的 IgM 抗体，使用了 SARS-CoV-2 双抗原多物种诊断酶联免疫吸附测定试剂盒。通过测量血清中的 IgG 抗体滴度来确认和验证血清阳性样本。登记的动物来自埃及吉萨省的不同地点，并在大流行期间采样；其中包括 92 只伴侣动物和 92 头家骆驼。研究发现，4.76%（1/21 临床样本）的狗、7.69% 的猫（1/13 庇护所样本）和 1.08%（1/92）的骆驼具有可测量的 SARS-CoV-2 NP IgM 抗体。所有 IgM 血清阳性样本均为 IgG 阳性，狗、猫和骆驼的可测量滴度分别为 34.5、28.6 和 25.8 UI/mL。据我们所知，这项研究是第一个评估在埃及调查的特定动物中 SARS-CoV-2 血清流行率的研究。这些结果可能预示着宠物和骆驼在 SARS-CoV-2 病毒维持中具有良好的流行病学作用。因此，我们的研究结果应该通过全国性血清流行率研究得到证实，并且需要进一步的研究来澄清这些动物是主动还是被动携带者。