Soybean is one of the chief crops producing protein and oil for human consumption. Wild soybean, the ancestor of cultivated soybean, possesses high seed protein content; therefore, it is a valuable genetic resource that could enhance protein content in the cultivated varieties. To identify the genes responsible for increasing protein content in wild soybean, a population comprising 113 BC4F6 chromosome segment substitution lines (CSSL) was developed from a cross between soybean cultivar ‘Jackson’ and wild soybean accession JWS156-1. The CSSL population was cultivated in the field conditions for 3 years (2018, 2019 and 2020), and the seeds harvested from each line were analysed for protein and oil contents by InfraTec Nova instrument. Quantitative trait locus (QTL) analysis with 243 SSR markers identified 12 QTLs associated with seed protein, oil and protein + oil contents. Among these QTLs, qPro8 and qPro19, two major and stable QTLs for protein content, were detected on chromosomes 8 and 19, respectively. No QTL for oil content was detected in the vicinity of qPro19, indicating that qPro19 did not influence the seed oil content. The effect of qPro19 was validated using near-isogenic lines (NILs) of qPro19. By introducing the qPro19 allele from wild soybean into another soybean variety, ‘Tachiyutaka’, a BC4 line, T-678, that showed enhanced seed protein content, without reducing the seed oil content. This study implied that the qPro19 allele from wild soybean could be a potential genetic resource for breeding programmes aimed to improve soybean seed quality.

大豆是生产人类消费蛋白质和油的主要作物之一。野生大豆是栽培大豆的祖先，种子蛋白质含量高；因此，它是一种宝贵的遗传资源，可以提高栽培品种的蛋白质含量。为了鉴定导致野生大豆蛋白质含量增加的基因，该群体包含 113 BC4F64等位基因可能是旨在提高大豆种子质量的育种计划的潜在遗传资源。qPro19 品系 T-678，显示出种子蛋白质含量提高，但种子油含量不降低。这项研究表明，来自野生大豆的等位基因引入另一个大豆品种“Tachiyutaka”（BC）qPro19qPro19 的效果已使用 qPro19不影响种子含油量。 qPro19表明qPro19附近未检测到含油量QTL，两个主要且稳定的QTL蛋白质含量分别在8号和19号染色体上检测。 qPro19和qPro8染色体片段替换系(CSSL)是由大豆品种“Jackson”和野生大豆编号JWS156-1之间的杂交开发的。 CSSL群体在田间条件下培育3年（2018年、2019年和2020年），并通过InfraTec Nova仪器分析从每个品系收获的种子的蛋白质和油含量。使用 243 个 SSR 标记进行数量性状位点 (QTL) 分析，鉴定出 12 个与种子蛋白质、油和蛋白质 + 油含量相关的 QTL。其中，