Due to high antimicrobial resistance and biofilm-forming ability, Pseudomonas aeruginosa is one of the seriously life-threatening agents causing chronic and nosocomial infections. This study was performed to determine the antibiotic resistance pattern, biofilm formation, and frequency of biofilm-related genes in P. aeruginosa strains. In total, 123 P. aeruginosa isolates were collected from different clinical sources. Antimicrobial susceptibility testing (AST) was performed to detect multidrug-resistant P. aeruginosa (MDRPA) isolates. To evaluate the biofilm-forming isolates, the microtiter plate (MTP) method was carried out. Also, the prevalence of biofilm genotype patterns, including pslA, pslD, pelA, pelF, and algD genes, was detected by polymerases chain reaction (PCR). According to our findings, the highest resistance and susceptibility rates were found in ceftazidime with 74.7% (n = 92) and ciprofloxacin with 42.2% (n = 52), respectively. In our study, the highest level of antibiotic resistance belonged to wound isolates which meropenem had the most antibacterial activity against them. In total, 86.1% (n = 106) P. aeruginosa isolates were determined as MDRPA, of which 61.3% (n = 65) were able to form strong biofilm. The highest and lowest frequency of biofilm-related genes among biofilm producer isolates belonged to pelF with 82.1% (n = 101) and algD with 55.2% (n = 68), respectively. The findings of the conducted study indicate a significant relationship between MDRPA and biofilm genotypic/phenotypic patterns, suggesting the necessity of a careful surveillance program in hospital settings.

由于具有较高的抗菌耐药性和生物膜形成能力，铜绿假单胞菌是导致慢性和医院感染的严重危及生命的病原体之一。本研究旨在确定铜绿假单胞菌菌株中的抗生素耐药性模式、生物膜形成以及生物膜相关基因的频率。总共从不同的临床来源收集了123 株铜绿假单胞菌分离株。进行抗菌药物敏感性测试 (AST) 来检测多重耐药铜绿假单胞菌(MDRPA) 分离株。为了评估生物膜形成分离株，采用微量滴定板（MTP）方法。此外，还通过聚合酶链式反应 (PCR) 检测了生物膜基因型模式的流行情况，包括pslA、pslD、pelA、pelF和algD基因。根据我们的研究结果，头孢他啶的耐药率和敏感性最高，分别为 74.7% ( n = 92) 和环丙沙星，为 42.2% ( n = 52)。在我们的研究中，抗生素耐药性最高的是伤口分离株，美罗培南对其具有最强的抗菌活性。总共，86.1% ( n = 106)的铜绿假单胞菌分离株被确定为 MDRPA，其中 61.3% ( n = 65) 能够形成牢固的生物膜。生物膜生产菌株中生物膜相关基因频率最高和最低的是pelF，分别为82.1% ( n = 101) 和algD ，分别为 55.2% ( n = 68)。所进行的研究结果表明 MDRPA 与生物膜基因型/表型模式之间存在显着关系，表明在医院环境中进行仔细监测计划的必要性。