The interaction of mesenchymal stromal cells (MSC) in co-cultures with hematopoietic progenitor cells (HPC) in ex-vivo models induce the secretion of pro-inflammatory protein factors and influences HPC cell proliferation and differentiation processes. Different studies have shown the quantification of these biomarkers in other conditioned media using an ELISA and Luminex™ type immunoassay format. In this paper, we developed real-time quantification of biomarkers in conditioned media of these cells in co-cultures from bone marrow, umbilical cord blood, and Wharton's jelly sources using surface plasmon resonance imaging (SPRi). We show the detection and quantification of CCL2, CCL5, bFGF, IL-6, IL-8 and VEGF proteins with high sensitivity and specificity. SPRi can be used with a real-time analysis methodology and could be considered a tool for biomarker assays.

中文翻译：

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与造血祖细胞共培养的间充质基质细胞 (MSC) 条件培养基分泌的蛋白质的实时定量

在离体模型中，间充质基质细胞（MSC）与造血祖细胞（HPC）共培养时相互作用，诱导促炎蛋白因子的分泌，并影响HPC细胞的增殖和分化过程。不同的研究表明，使用 ELISA 和 Luminex™ 型免疫测定形式可以对其他条件培养基中的这些生物标志物进行定量。在本文中，我们使用表面等离子体共振成像（SPRi）对来自骨髓、脐带血和沃顿胶源的共培养物中的这些细胞的条件培养基中的生物标志物进行实时定量。我们展示了 CCL2、CCL5、bFGF、IL-6、IL-8 和 VEGF 蛋白的高灵敏度和特异性检测和定量。 SPRi 可与实时分析方法一起使用，并可被视为生物标志物测定的工具。