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Genome size and DNA endoreplication pattern of non-generative cells in the antheridia of Chara connivens Salzmann ex A. Braun from Northern Morocco
Flora ( IF 1.9 ) Pub Date : 2023-11-25 , DOI: 10.1016/j.flora.2023.152421
G.M. Gargiulo , R. Damino , A. Merzouki , R.M. Picone , F.Z. Redouan , A. Boutahar , A. Crisafulli

Data on nuclei, mitotic divisions, chromosomal behaviors, and DNA measurements have been examined in individuals of Chara connivens collected in a pond from the Tangier-Tetouan Region (Northern Morocco). Image Cytometry of individual nuclei was used to examine the endoreduplication level in germ line and somatic cells of the antheridia. DNA content increased from 2C up to 128C in shield cells, manubria and capitular cells. However, a progressive decrease in the amount of DNA after any successive endoreplication cycle was apparent in the antheridial non-generative cells suggesting a possible presence of a DNA remodeling mechanism in Chara. The 1C-value found in the cells of the filaments at a mature stage of spermiogenesis has an absolute content of 4.47 pg. The obtained data on DNA amount was combined and analyzed with those available on Chara spp. in the literature. An n = 14 chromosome number was confirmed for this population. Spontaneous anomalies such as laggard chromosomes, formation of micronuclei or amitotic nuclear divisions by gradually pulling apart the nucleus into two parts, were observed in some cells of the antheridial filaments.



中文翻译:

摩洛哥北部 Chara connivens Salzmann ex A. Braun 花药非生殖细胞的基因组大小和 DNA 内复制模式

有关细胞核、有丝分裂、染色体行为和 DNA 测量的数据已在丹吉尔-得土安地区(摩洛哥北部)池塘中收集的Chara connivens个体中进行了检查。使用单个细胞核的图像细胞计数来检查生殖系和花药体细胞的核内复制水平。盾细胞、柄细胞和头状细胞中的 DNA 含量从 2℃ 增加到 128℃。然而,在任何连续的内复制周期后,在雄虫非生殖细胞中,DNA 量明显减少,这表明轮藻中可能存在 DNA 重塑机制。在精子发生成熟阶段的细丝细胞中发现的1C-值的绝对含量为4.47pg。将获得的 DNA 量数据与轮藻属 ( Chara spp ) 上可用的数据结合起来并进行分析。在文献中。 该群体的染色体数已确认为n = 14。在花药丝的一些细胞中观察到自发异常,例如落后染色体、微核的形成或通过逐渐将细胞核分成两部分而形成的无丝分裂核分裂。

更新日期:2023-11-29
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