The use of medicinal plants exists before the existence of humans. Healing with herbal treatments is of utmost significance because medicinal plants contain bioactive compounds. The objective of the current investigation was to evaluate the biological potential of methanolic (PAM) and chloroform (PAC) extracts of Portulacaria afra root, leaf, seed and flower. FT-IR (Fourier Transform Infrared Spectroscopy) spectroscopy was completed to detect the different functional groups. total antioxidant capacity (TAC), total reducing power (TRP) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays, were done for the determination of antioxidant potential of medicinal plant P. afra. Five different bacterial strains were used to evaluate the antibacterial potential using disc diffusion method. Cytotoxicity assay was used to test the cytotoxic potential against brine shrimps and shown the maximum cytotoxic potential with lowest IC₅₀ value (41.41 ± 0.80 µg/mL) in PAM. Radish seed germination phytotoxicity assay was performed and revealed the highest phytotoxic potential in PAM extract. ANOVA was applied via statistics version-8.1, and experimentations were done in triplicates. Maximum DPPH scavenging potential was observed in methanol extract of P. afra (PAM). The PAM extract has the highest flavonoid concentration (30.70 mg/g). PAM demonstrated maximum total reducing power (73.46 gallic acid equivalents per gram (GAE/g). Antibacterial activity was performed against different bacterial strains and PAM shown the best antibacterial activity with largest zone of inhibition (17 mm) contrary to Pseudomonas aeruginosa. The bioactive compounds found in P. afra extracts have antibacterial, antioxidant, and cytotoxic properties, revealing their ability to induce cell death in cancerous cell lines, indicating potential anticancer agents and making them intriguing candidates for future research and the possible creation of new therapeutics. we advise the use of these extracts against biofilms as these extracts have shown significant antibacterial activity against gram negative bacteria“.

药用植物的使用早在人类出现之前就已存在。草药治疗至关重要，因为药用植物含有生物活性化合物。当前研究的目的是评估马齿苋根、叶、种子和花的甲醇 (PAM) 和氯仿 (PAC) 提取物的生物潜力。完成FT-IR（傅里叶变换红外光谱）光谱以检测不同的官能团。采用总抗氧化能力 (TAC)、总还原力 (TRP) 和2, 2-二苯基-1-三硝基苯肼(DPPH) 测定来测定药用植物P. afra的抗氧化潜力。使用纸片扩散法使用五种不同的细菌菌株来评估抗菌潜力。采用细胞毒性测定法测试了 PAM对丰年虾的细胞毒性潜力，结果显示 PAM 具有最大的细胞毒性潜力和最低的 IC _{50值 (41.41 ± 0.80 µg/mL)。}进行了萝卜种子发芽植物毒性测定，结果显示 PAM 提取物具有最高的植物毒性潜力。通过统计版本 8.1 应用方差分析，并一式三份进行实验。在P. afra (PAM)的甲醇提取物中观察到最大 DPPH 清除潜力。PAM 提取物具有最高的类黄酮浓度（30.70 mg/g）。PAM 表现出最大的总还原能力（73.46 没食子酸当量/克 (GAE/g)）。对不同的菌株进行了抗菌活性，与铜绿假单胞菌相比，PAM 表现出最佳的抗菌活性，具有最大的抑菌圈 (17 mm) 。P. afra提取物中发现的化合物具有抗菌、抗氧化和细胞毒性特性，揭示了它们诱导癌细胞系细胞死亡的能力，表明了潜在的抗癌药物，并使它们成为未来研究和可能创造新疗法的有趣候选者。使用这些提取物对抗生物膜，因为这些提取物对革兰氏阴性菌表现出显着的抗菌活性“。