The probable synergistic effect of chemically synthesized zinc oxide nanoparticles and locally secured Moringa oliefera was assessed on diabetic rats’ model along with a potential effect on the male reproductive system and rat male serological markers. The plant M. oliefera was procured from the University of Agriculture Faisalabad step solvothermal method was utilized to synthesize the zinc oxide nanoparticles. The zeta sizer, UV–vis, and spectroscopic analysis of ZnO nanoparticles were undertaken. A total of 50 healthy male albino Wistar rats weighing 250 ± 10 g were used in the experimental research study having were divided into six stops designated as negative control, positive control (PC), standard control (Std. C), ZnO nanoparticle group (ZnO), M. oliefera Group (MO), and a group of combinations thereof (ZnONP + MO). The mean body weight was observed to be significantly normalized in group ZnONP + MO, i.e., 230 ± 6 g in contrast to PC, i.e., 162 ± 4 g; . The ZnONP + MO combination had a normoglycemic effect, i.e., 154.4 ± 4.5 mg/dl as in opposition to the PC, i.e., 315.7 ± 3 mg/dl. The serum level of rat testosterone in the ZnONP + MO group was observed at 0.958 ± 0.08 ng/ml in opposition to PC, which revealed it at 0.442 ± 0.02 ng/ml. The follicle-stimulating hormone level in the ZnONP + MO group was recorded at 10.04 ± 0.04 mlU/ml significantly varied from the PC, whose level was noted at 5.08 ± 0.09 mlU/ml. The level of LH in the ZnONP + MO group was observed at 6.89 ± 0.08 mIU/ml, significantly different from PC at 3.78 ± 0.08 mIU/ml. Histopathological changes in the rat testes treated with alloxan alone revealed the distortion in the epithelium of seminiferous tubules (H&E stain). However, the histopathology of testes isolated from rats treated with the zinc oxide nanoparticle and M. oliefera combination showed almost normal spermatogenic activity, the lumen of seminiferous tubules contained sperms, normal spermatids, and outer epithelium layer of seminiferous tubules was intact.

中文翻译：

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氧化锌纳米颗粒和辣木补充剂对糖尿病大鼠模型雄性生殖系统的理化评估

在糖尿病大鼠模型上评估了化学合成的氧化锌纳米粒子和局部固定的辣木可能的协同效应，以及对雄性生殖系统和大鼠雄性血清学标志物的潜在影响。植物M. oliefera购自费萨拉巴德农业大学，采用一步溶剂热法合成氧化锌纳米颗粒。对 ZnO 纳米颗粒进行了 Zeta 粒度测定、紫外可见分光光度计和光谱分析。实验研究选用健康雄性白化Wistar大鼠50只，体重250±10g，分为6组，分别为阴性对照、阳性对照（PC）、标准对照（Std.C）、ZnO纳米颗粒组（ ZnO)、M. oliefera Group (MO) 及其组合 (ZnONP + MO)。观察到 ZnONP + MO 组的平均体重显着标准化，即 230 ± 6 g，而 PC 组为 162 ± 4 g；。ZnONP+MO组合具有正常血糖作用，即154.4±4.5mg/dl，而PC则为315.7±3mg/dl。ZnONP + MO 组大鼠血清睾酮水平为 0.958 ± 0.08 ng/ml，而 PC 组为 0.442 ± 0.02 ng/ml。ZnONP + MO 组的卵泡刺激素水平为 10.04 ± 0.04 mIU/ml，与 PC 组的卵泡刺激素水平显着不同，PC 组的卵泡刺激素水平为 5.08 ± 0.09 mIU/ml。ZnONP + MO 组的 LH 水平为 6.89 ± 0.08 mIU/ml，与 PC 的 3.78 ± 0.08 mIU/ml 显着不同。仅用四氧嘧啶处理的大鼠睾丸的组织病理学变化揭示了生精小管上皮的变形（H＆E染色）。然而，用氧化锌纳米粒子和油橄榄组合治疗的大鼠分离的睾丸的组织病理学显示几乎正常的生精活性，生精小管的管腔含有精子，精子细胞正常，并且生精小管的外上皮层完整。