Knowledge of the plant proteins and metabolites synthesized in response to Ganoderma boninense infection of oil palm is useful for the development of planting materials resistant to the basal stem rot (BSR) disease. Breeding for disease-resistant oil palm progenies is an effective way of adapting to the spread of BSR. In this study, TUP 1281 and TUP 1309, partial resistant and susceptible oil palm progenies, respectively, were artificially inoculated with G. boninense. The molecular responses in the root and spear leaf tissues, following exposure to the fungus, were examined using liquid chromatography-mass spectrometry (LC-MS)-based proteomics and metabolomics approaches. During infection, some of the most enriched pathways in the partial resistant progeny were the biosynthesis of unsaturated fatty acids (UFAs), the biosynthesis of secondary metabolites, and fatty acid metabolism and its elongation. Meanwhile in the susceptible progeny, the fatty acid elongation pathways were found to be reduced. Accumulation of chelidonic acid in both the partial resistant and susceptible oil palm progenies after inoculation was also observed, indicating the metabolite's involvement in response to G. boninense infection. Our results showed that the TUP1281 and TUP1309 possess distinctive proteins and metabolites that exhibit different biochemical responses, which could likely explain the resistance towards G. boninense.

了解针对油棕博宁灵芝感染而合成的植物蛋白和代谢物对于开发抗基部茎腐病（BSR）病害的种植材料非常有用。培育抗病油棕后代是适应 BSR 蔓延的有效途径。在这项研究中，TUP 1281 和 TUP 1309（分别是部分抗性和敏感油棕后代）用G. boninense人工接种。使用基于液相色谱-质谱（LC-MS）的蛋白质组学和代谢组学方法检查了接触真菌后根和矛叶组织的分子反应。在感染过程中，部分抗性后代中最丰富的一些途径是不饱和脂肪酸（UFA）的生物合成、次生代谢物的生物合成以及脂肪酸代谢及其延伸。同时，在易感后代中，发现脂肪酸延伸途径减少。接种后，还观察到部分抗性和易感性油棕后代中白屈菜酸的积累，表明该代谢物参与了对博宁G. boninense感染的反应。我们的结果表明，TUP1281 和 TUP1309 具有独特的蛋白质和代谢物，表现出不同的生化反应，这可能解释了对G. boninense的抗性。