The sleepy cod Oxyeleotris lineolata is native to northern Australia. At present, there is little information on the genome information of the sleepy cod. In this study, a total of 48 novel single nucleotide polymorphism (SNP) markers were developed based on whole-genome resequencing. The analysis of the 48 SNP genotypes in 58 fish showed that the observed heterozygosity and expected heterozygosity ranged from 0.3030 to 0.6667 and 0.2133 to 0.8541, respectively. The minor allele frequency ranged from 0.1211 to 0.4899, and the polymorphism information content ranged from 0.1902 to 0.3749. Among these SNPs, seven SNPs were found to deviate from Hardy–Weinberg equilibrium significantly (P < 0.05). These SNP markers could find application in genetic linkage maps and association analysis with growth traits of sleepy cod.

昏昏欲睡的鳕鱼Oxyeleotris lineolata原产于澳大利亚北部。目前，关于瞌睡鳕鱼的基因组信息还很少。在这项研究中，基于全基因组重测序，总共开发了 48 个新型单核苷酸多态性 (SNP) 标记。对58条鱼的48个SNP基因型的分析表明，观测杂合度和预期杂合度分别为0.3030至0.6667和0.2133至0.8541。次要等位基因频率范围为0.1211～0.4899，多态性信息含量范围为0.1902～0.3749。在这些SNP中，有7个SNP显着偏离Hardy-Weinberg平衡（P  < 0.05）。这些 SNP 标记可应用于遗传连锁图谱以及与瞌睡鳕鱼生长性状的关联分析。