Ovarian cancer (OC) is a common malignancy in women of reproductive age. Circular RNAs (circRNAs) are emerging players in OC progression. We investigated the function and mechanism of circular RNA hsa_circ_0027803 (circCDK17) in OC pathogenesis. RT-qPCR and western blot were utilized for gene and protein expression analysis, respectively. CCK-8, EdU and Transwell assays investigated OC cell proliferation, migration and invasion. The associations between circCDK17, miR-22-3p and CD147 were examined by dual luciferase reporter and RIP assays. The in vivo model of OC nude mice was constructed to explore the role of circCDK17. CircCDK17 was increased in OC tissue and cells, and patients with higher expression of circCDK17 had a shorter survival. CircCDK17 downregulation inhibited OC cell proliferation, migration and invasion, and reduced epithelial-mesenchymal transition (EMT)-related markers. In vivo experiments showed that circCDK17 silencing inhibited OC tumor growth and metastasis. CircCDK17 depletion reduced CD147 level via sponging miR-22-3p. MiR-22-3p knockdown overturned effect of circCDK17 depletion on OC cell proliferation, migration and invasion. Meanwhile, overexpressed CD147 restored functions of circCDK17 downregulation on OC development. CircCDK17 is an important molecule that regulates OC pathogenic process through miR-22-3p/CD147.

中文翻译：

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CircCDK17通过海绵miR-22-3p调节CD147表达来促进卵巢癌细胞的增殖和转移。

卵巢癌（OC）是育龄妇女常见的恶性肿瘤。环状 RNA (circRNA) 是 OC 进展中的新兴参与者。我们研究了环状 RNA hsa_circ_0027803 (circCDK17) 在 OC 发病机制中的功能和机制。RT-qPCR 和蛋白质印迹分别用于基因和蛋白质表达分析。CCK-8、EdU 和 Transwell 检测研究了 OC 细胞增殖、迁移和侵袭。通过双荧光素酶报告基因和 RIP 测定来检查 circCDK17、miR-22-3p 和 CD147 之间的关联。构建OC裸鼠体内模型，探讨circCDK17的作用。OC组织和细胞中CircCDK17增加，circCDK17表达较高的患者生存期较短。CircCDK17 下调可抑制 OC 细胞增殖、迁移和侵袭，并减少上皮间质转化 (EMT) 相关标志物。体内实验表明，circCDK17沉默可抑制OC肿瘤的生长和转移。CircCDK17 耗竭通过海绵 miR-22-3p 降低 CD147 水平。MiR-22-3p 敲低推翻了 circCDK17 耗竭对 OC 细胞增殖、迁移和侵袭的影响。同时，过表达的 CD147 恢复了 circCDK17 下调对 OC 发育的功能。CircCDK17是通过miR-22-3p/CD147调节OC致病过程的重要分子。