Enterobacter species represent widely distributed opportunistic pathogens, commonly associated with plants and humans. In the present study, we performed a detailed molecular characterization as well as genomic study of a type VI secretion system (T6SS) bacterium belonging to member of the family Enterobacteriaceae and named Enterobacter sp. S-33. The comparative sequence analysis of the 16S rRNA gene showed that the strain was closely related to other Enterobacter species. The complete genome of the strain with a genome size of 4.6 Mbp and GC-content of 55.63% was obtained through high-quality sequencing. The genomic analysis with online tools unravelled the various genes belonging to the bacterial secretion system, antibiotic resistance, virulence, efflux pumps, etc. The isolate showed the motility behavior that contributes to Enterobacter persistence in a stressed environment and further supports infections. PCR amplification and further sequencing confirmed the presence of drug-efflux genes acrA, acrB, and outer membrane genes, viz. OmpA, OmpC, and OmpF. The cell surface hydrophobicity and co-aggregation assay against different bacterial strains illustrated its putative pathogenic nature. Genome mining identified various biosynthetic gene clusters (BGCs) corresponding to non-ribosomal proteins (NRPS), siderophore, and arylpolyene production. Briefly, genome sequencing and detailed characterization of environmental Enterobacter isolate will assist in understanding the epidemiology of Enterobacter species, and the further prevention and treatment of infectious diseases caused by these broad-host range species.

肠杆菌属广泛分布的机会性病原体，通常与植物和人类有关。在本研究中，我们对属于肠杆菌科成员并命名为肠杆菌属的 VI 型分泌系统 (T6SS) 细菌进行了详细的分子表征和基因组研究。S-33。16S rRNA基因比较序列分析表明该菌株与其他肠杆菌属密切相关。通过高质量测序获得该菌株全基因组，基因组大小为4.6 Mbp，GC含量为55.63%。使用在线工具进行的基因组分析揭示了属于细菌分泌系统、抗生素耐药性、毒力、外排泵等的各种基因。分离株显示出有助于肠杆菌在应激环境中持续存在并进一步支持感染的运动行为。PCR 扩增和进一步测序证实了药物流出基因 acrA、acrB 和外膜基因的存在，即。OmpA、OmpC 和 OmpF。针对不同细菌菌株的细胞表面疏水性和共聚集测定说明了其假定的致病性质。基因组挖掘确定了与非核糖体蛋白 (NRPS)、铁载体和芳基多烯生产相对应的各种生物合成基因簇 (BGC)。简而言之，环境肠杆菌分离株的基因组测序和详细表征将有助于了解肠杆菌物种的流行病学，以及进一步预防和治疗由这些广泛宿主范围物种引起的传染病。