Zika virus can infect the fetus through the placental barrier, causing ZIKV congenital syndrome and even miscarriage, which can cause great harm to pregnant women and infants. Currently, there is no vaccine and drug available to combat the Zika virus. In this study, we designed a fusion protein named EDIII-Fc, including the EDIII region of Zika E protein and human IgG Fc fragment, and obtained 293T cells that stably secreted EDIII-Fc protein using the lentiviral expression system. Mice were immunized with the EDIII-Fc protein, and it was observed that viral replication was significantly inhibited in the immunized mice compared to non-immunized mice. In rhesus macaques, we found that EDIII-Fc effectively induce the secretion of neutralizing antibodies and T cell immunity. These experimental data provide valid data for further use of Zika virus E protein to prepare an effective, safe, affordable Zika vaccine.

中文翻译：

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EDIII-Fc 在小鼠和恒河猴中诱导针对寨卡病毒的保护性免疫反应。

寨卡病毒可通过胎盘屏障感染胎儿，引起寨卡病毒先天综合征甚至流产，对孕妇和婴儿造成极大危害。目前，尚无疫苗和药物可用于对抗寨卡病毒。在本研究中，我们设计了一种名为EDIII-Fc的融合蛋白，包括Zika E蛋白的EDIII区域和人IgG Fc片段，并利用慢病毒表达系统获得了稳定分泌EDIII-Fc蛋白的293T细胞。用EDIII-Fc蛋白免疫小鼠，观察到与未免疫小鼠相比，免疫小鼠的病毒复制受到显着抑制。在恒河猴中，我们发现EDIII-Fc有效诱导中和抗体的分泌和T细胞免疫。这些实验数据为进一步利用寨卡病毒E蛋白制备有效、安全、实惠的寨卡疫苗提供了有效数据。