Rhodotorula toruloides is being developed for the use in industrial biotechnology processes because of its favorable physiology. This includes its ability to produce and store large amounts of lipids in the form of intracellular lipid bodies. Nineteen strains were characterized for mating type, ploidy, robustness for growth, and accumulation of lipids on inhibitory switchgrass hydrolysate (SGH). Mating type was determined using a novel polymerase chain reaction (PCR)-based assay, which was validated using the classical microscopic test. Three of the strains were heterozygous for mating type (A1/A2). Ploidy analysis revealed a complex pattern. Two strains were triploid, eight haploid, and eight either diploid or aneuploid. Two of the A1/A2 strains were compared to their parents for growth on 75%v/v concentrated SGH. The A1/A2 strains were much more robust than the parental strains, which either did not grow or had extended lag times. The entire set was evaluated in 60%v/v SGH batch cultures for growth kinetics and biomass and lipid production. Lipid titers were 2.33-9.40 g/L with a median of 6.12 g/L, excluding the two strains that did not grow. Lipid yields were 0.032-0.131 (g/g) and lipid contents were 13.5-53.7% (g/g). Four strains had significantly higher lipid yields and contents. One of these strains, which had among the highest lipid yield in this study (0.131 ± 0.007 g/g), has not been previously described in the literature. SUMMARY The yeast Rhodotorula toruloides was used to produce oil using sugars extracted from a bioenergy grass.

中文翻译：

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确定圆红酵母的交配类型和倍性及其对木质纤维素生物质糖生长的影响。

由于其良好的生理学特性，圆红酵母正在被开发用于工业生物技术过程。这包括其以细胞内脂质体的形式产生和储存大量脂质的能力。对 19 个菌株的交配类型、倍性、生长稳健性以及抑制性柳枝稷水解产物 (SGH) 上的脂质积累进行了表征。使用基于聚合酶链反应（PCR）的新型测定法确定交配类型，并使用经典的显微镜测试对其进行了验证。其中三个菌株对于交配类型而言是杂合的（A1/A2）。倍性分析揭示了一种复杂的模式。两个菌株是三倍体，八个是单倍体，八个是二倍体或非整倍体。将两个 A1/A2 菌株与其亲本在 75%v/v 浓缩 SGH 上的生长情况进行比较。A1/A2 菌株比亲本菌株坚固得多，亲本菌株要么不生长，要么滞后时间延长。整个组在 60%v/v SGH 分批培养物中进行生长动力学、生物量和脂质生产的评估。排除不生长的两种菌株，脂质滴度为2.33-9.40 g/L，中位数为6.12 g/L。脂质产量为0.032-0.131(g/g)，脂质含量为13.5-53.7%(g/g)。4个菌株的脂质产量和含量显着较高。其中一种菌株在本研究中具有最高的脂质产量（0.131 ± 0.007 g/g），之前的文献中尚未描述过。摘要 酵母红酵母用于利用从生物能源草中提取的糖来生产油。